Gi Min

写真a

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Title

Associate Professor

Laboratory location

Abeno Campus

Research Areas 【 display / non-display

Experimental Pathology, Hominal Pathology

Research Interests 【 display / non-display

Experimental animal model, chemical carcinogenesis, cancer chemoprevention, Urinary bladder carcinogenesis

Research Career 【 display / non-display

  • chemical carcinogenesis

    (Collaboration in Organization) Project Year :

    1997.04
    -
    Today

    Keyword in research subject:  Chemical carcinogenesis, Cancer risk assessment, Arsenic

  • Cancer chemoprevention

    (Collaboration in Organization) Project Year :

    1997.04
    -
    Today

    Keyword in research subject:  Urinary bladder cancer, Liver cancer, Colon cancer

  • Molecular carcinogenesis

    (Collaboration in Organization) Project Year :

    1996.04
    -
    Today

    Keyword in research subject:  Urinary bladder, Biomarker for carcinogenesis, microRNA

Association Memberships 【 display / non-display

  • Japanese Cancer Association

  • The Japanese Society of Pathology

  • The Japanese Society of Toxicologic Pathology

  • American Association for Cancer Rsearch

  • Japanese Association of Cancer Prevention

Current Career 【 display / non-display

  • Osaka City University   Graduate School of Medicine   Basic Medicine Course   Associate Professor  

Graduate School 【 display / non-display

  •  
    -
    2001

    Osaka City University  Graduate School, Division of Medicine  pathology 

Graduating School 【 display / non-display

  •  
    -
    1993

    SHENYANG MEDICAL COLLEGE   Faculty of Medicine  

 

Published Papers 【 display / non-display

  • Cache Domain Containing 1 Is a Novel Marker of Non-Alcoholic Steatohepatitis-Associated Hepatocarcinogenesis.

    Kakehashi A, Chariyakornkul A, Suzuki S, Khuanphram N, Tatsumi K, Yamano S, Fujioka M, Gi M, Wongpoomchai R, Wanibuchi H

    Cancers  13 ( 6 )  2021.03  [Refereed]

    DOI PubMed

  • Cell proliferation of rat bladder urothelium induced by nicotine is suppressed by the NADPH oxidase inhibitor, apocynin.

    Suzuki S, Cohen SM, Arnold LL, Pennington KL, Gi M, Kato H, Naiki T, Naiki-Ito A, Wanibuchi H, Takahashi S

    Toxicology letters  336   32 - 38 2021.01  [Refereed]

    DOI PubMed

  • Accumulation of 8-hydroxydeoxyguanosine, L-arginine and Glucose Metabolites by Liver Tumor Cells Are the Important Characteristic Features of Metabolic Syndrome and Non-Alcoholic Steatohepatitis-Associated Hepatocarcinogenesis.

    Kakehashi A, Suzuki S, Ishii N, Okuno T, Kuwae Y, Fujioka M, Gi M, Stefanov V, Wanibuchi H

    International journal of molecular sciences  21 ( 20 )  2020.10  [Refereed]

    DOI PubMed

  • Role of γ-H2AX as a biomarker for detection of bladder carcinogens in F344 rats.

    Suzuki S, Gi M, Toyoda T, Kato H, Naiki-Ito A, Kakehashi A, Ogawa K, Takahashi S, Wanibuchi H

    Journal of toxicologic pathology  33 ( 4 ) 279 - 285 2020.10  [Refereed]

    DOI PubMed

  • Myeloid-derived suppressor cells are essential partners for immune checkpoint inhibitors in the treatment of cisplatin-resistant bladder cancer.

    Takeyama Y, Kato M, Tamada S, Azuma Y, Shimizu Y, Iguchi T, Yamasaki T, Gi M, Wanibuchi H, Nakatani T

    Cancer letters  479   89 - 99 2020.06  [Refereed]

    DOI PubMed

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Review Papers (Misc) 【 display / non-display

  • 【環境因子と発がん】化学物質曝露による職業がんの発生要因とメカニズム 動物実験を基礎とした新たな職業性膀胱がん原因物質の発見

    鰐渕 英機, 魏 民, 鈴木 周五

    (株)羊土社 実験医学  38 ( 11 ) 1842 - 1848 2020.07

     View Summary

    職業に起因する発がん要因に曝露され生じるがんを職業がんといい、産業の発達とともに大きな問題となっている。職業性膀胱がんは主に染料産業に発生し、芳香族アミンが主な原因物質として報告されている。日本でも2019年にオルトトルイジンにさらされる業務による膀胱がんが、厚生労働省の「職業病リスト」に記載され、労災補償の対象となり話題となった。われわれは同工場で生産されていた新規芳香族アミンの発がん性およびそのメカニズムを、動物実験を介して解明した。ここでは、職業がんの歴史から発がん物質の検出法、動物実験モデルの有効性について解説したい。(著者抄録)

  • 【"ヒ素"とは何か】発がん物質としてのヒ素

    鈴木 周五, 魏 民, 鰐渕 英機

    (株)杏林書院 保健の科学  62 ( 6 ) 374 - 379 2020.06

  • 膀胱癌の実験モデルにおけるleucineとisoleucinの役割(Roles of Leucine and Isoleucine in Experimental Models of Bladder Carcinogenesis)

    Gi Min, Wanibuchi Hideki

    内閣府食品安全委員会 Food Safety  3 ( 4 ) 136 - 142 2015.12  [Refereed]  [Invited]

  • 化学発がん物質のリスク評価における閾値問題

    福島 昭治, 魏 民, 梯 アンナ, 鰐渕 英機

    (一社)日本毒性学会 The Journal of Toxicological Sciences  39 ( Suppl. ) S15 - S15 2014.07  [Refereed]  [Invited]

  • 発がん物質にも閾値が存在する

    福島 昭治, 魏 民, 梯 アンナ, 鰐渕 英機

    日本マイコトキシン学会 マイコトキシン  58 ( 2 ) 119 - 128 2008.10  [Refereed]  [Invited]

     View Summary

    2-amino-3,8-dimethylimidazo[4,5-f] quinoxaline(MeIQx)は、魚や肉などの焼けこげに含まれている物質である。今回、MeIQxのラット肝臓における低用量発がん性を中期発がん性試験法にて検討した。その結果、MeIQx-DNA付加体形成は微量からみられた。より高い用量で8-hydroxy-2'-deoxyquanosine形成、lacI遺伝子変異、イニシエーション活性等が誘発された。また、肝臓の前腫瘍性病変であるglutahione S-transferase placental(GST-P)陽性細胞巣は、更により高い用量で誘発された。N-ニトロソ化合物であるN-nitrosodiethylamineやN-nitrosodimethylamineでもGST-P陽性細胞巣の発生は微量では発生しなかった。更に、大腸発がん物質である2-amino-1methyl-6-phenylimidazo[4,5-b] pyridine(PhIP)のラット発がん性を検討した結果、大腸粘膜におけるPhIP-DNA付加体形成は微量から認められた。前腫瘍性病変の代替マーカーである変異クリプト巣は、高用量でのみ誘発された。非遺伝毒性肝発がん物質であるphenobarbitalは、GST-P陽性細胞巣の発生を高用量では増加させ、逆に低用量ではその発生を抑制した。以上の結果から、遺伝毒性発がん物質には閾値、少なくとも実際的な閾値が、また、非遺伝毒性発がん物質には真の閾値が存在することが示唆された。

    DOI CiNii

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Conference Activities & Talks 【 display / non-display

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Grant-in-Aid for Scientific Research 【 display / non-display

  • Effects of excessive intake of trace elements and westernized high-fat diet on senile dementia

    Project/Area Number : 26350163  Grant-in-Aid for Scientific Research(C) Partaker / Other

    Project Year :

    2014.04
    -
    2017.03
     

     View Summary

    Zinc (Zn) is an essential element but excess amount of Zn is known to cause neurotoxic effects. The risk of excessive intake of Zn is increased by intake from foods plus dietary supplements. We investigated the effects of chronic exposure to Zn on a step-through passive avoidance test (PA), a Y-maze test and a novel object recognition test (NOR) in aged mice. The behavioral tests indicated the possibilities that chronic exposure to Zn dose-dependently inhibited long-term memory and object recognition memory. The increased consumption of saturated fats in a westernized high-fat diet (HFD) contributes to neurodegenerative disease and senile dementia. We investigated whether HFD influences learning and memory deficits caused by chronic exposure to Zn in aged mice. The results of PA, NOR and a fear conditioning test showed that HFD feeding might result in an increase of object recognition memory and a decrease of long-term memory or context memory.

  • Study of mechanism of carcinogenesis by 1,2-dichloropropane using animal experiment model

    Project/Area Number : 26340042  Grant-in-Aid for Scientific Research(C) Partaker / Other

    Project Year :

    2014.04
    -
    2017.03
     

     View Summary

    In this study, 1,2-dichloropropane (1,2-DCP) and toxicity were investigated using an animal experiment model. As a result, bile duct carcinogenicity was not observed in mouse, rat and hamster in animal test with single exposure of 1,2-DCP and DCM. However, co-exposures of 1,2-DCP and DCM revealed a stronger liver carcinogenic potential compared to single exposure to 1,2-DCP. In addition, it was suggested that as a mechanism of tumor development, a variety of signaling pathways are activated by increased expression of cell proliferation-related factors.

  • Inhibitory effects of carbonic anhydrase 2 inhibitor on the invasion of urinary bladder cancer

    Project/Area Number : 25462499  Grant-in-Aid for Scientific Research(C) Representative

    Project Year :

    2013.04
    -
    2016.03
     

    Partaker : TAMADA Satoshi

     View Summary

    We have demonstrated that that carbonic anhydrase 2 (CA2) is an invasion-associated factor in urothelial carcinoma (UC). The purpose to the present study is to examine the inhibitory effects of CA inhibitor Acetazolamide (Ace) on the N-butyl-N-(hydroxybutyl)nitrosameine (BBN)-induced mouse urinary bladder carcinogenesis. We found that concurrent treatment with Ace and Cisplatin (Cis) significantly inhibited the development of BBN-induced UC. Moreover, muscle invasive UC were significantly decreased by the Ace alone and concurrent treatment with Ace and Cis. These findings indicate that CA could serve as a potential therapeutic molecular target for bladder cancers and suggest that CA inhibitors are useful for prevention of UC invasion.

  • Investigation of Mechanism of Action of Steroid Sulfatase in Bladder Cancer

    Project/Area Number : 24592406  Grant-in-Aid for Scientific Research(C) Partaker / Other

    Project Year :

    2012.04
    -
    2015.03
     

    Partaker : WEI Min, KATO Minoru

     View Summary

    we found out that steroid sulfatase (STS), an enzyme involved in androgen synthesis, is an important factor of invasion and prognosis of bladder cancer (BCa) patients. First, we demonstrated that the expression level of STS was significantly higher in muscle invasive BCa compared to non muscle-invasive BCa and the prognosis of STS positive Bca is significantly worse than that of STS negative BCa. Sedond, STS-knock down BCa cells showed less migration and invasion capability. Lastly, we showed that STS is relevant to epithelial to mesenchymal factors such as vimentin and ZEB1.
    In conclusion, we showed the possibility that STS is a novel target of BCa treatment.

  • Effects of excessive intake of trace elements on senile neuropathy and the development of a method for biological monitoring of trace elements

    Project/Area Number : 23500978  Grant-in-Aid for Scientific Research(C) Partaker / Other

    Project Year :

    2011
    -
    2013
     

    Partaker : GI Min, KAWAZOE Sadahiro, KITAMURA Mari, KIMATA Isao

     View Summary

    Trace elements, such as zinc (Zn), iron (Fe), cupper (Cu) and manganese (Mn) are essential elements but excess amounts of these elements are known to cause many neurodegenerative diseases. Our studies showed that some supplements of botanical or animal origin contained high concentrations of Zn, Fe, Cu and Mn. Our questionnaire survey suggested that the risk of excessive intake of the trace elements is caused by taking food plus botanical or animal origin supplements.
    We investigated the effects of chronic exposure to Mn, Zn and Cu on animal behavior tests, a step-through passive avoidance task, a Y-maze test and a novel object recognition test, in male or female adult rats. These behavioral tests indicated the possibilities that chronic exposure to Mn inhibited long-term memory and chronic exposure to Zn inhibited object recognition memory.

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Other external funds procured 【 display / non-display

  • Effects of excessive combined intake of trace elements and westernized high-fat diet on senile dementia

    System name: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C) (Commissioned Research)  Representative

    Project Year :

    2017.04
    -
    2020.03

    Yoshida Kaoru

     View Summary

    Our previous studies showed that the possibilities that chronic exposure to Zn dose-dependently inhibited long-term memory and object recognition memory by a step-through passive avoidance task (PA) and a novel object recognition test (NOR). In this study we investigated the effect of Zn administration combined with Mn, Cu or Fe on learning and memory in aged mice. Furthermore, we investigated whether high-fat diet (HFD) influences learning and memory impairment caused by chronic administration of Zn combined with the trace elements in aged mice. The results of PA, and NOR test showed that the administration of Zn combined with Cu enhanced the impairment in learning and memory, but the administration of Zn combined with Mn or Fe no longer showed the impairment in learning and memory. HFD feeding might result in decrease the impairment in learning and memory of mice caused by the administration of Zn combined with Cu.

  • Study of mechanism of carcinogenesis by 1,2-dichloropropane using animal experiment model

    System name: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C) (Commissioned Research)  Representative

    Project Year :

    2014.04
    -
    2017.03

    Wanibuchi Hideki

     View Summary

    In this study, 1,2-dichloropropane (1,2-DCP) and toxicity were investigated using an animal experiment model. As a result, bile duct carcinogenicity was not observed in mouse, rat and hamster in animal test with single exposure of 1,2-DCP and DCM. However, co-exposures of 1,2-DCP and DCM revealed a stronger liver carcinogenic potential compared to single exposure to 1,2-DCP. In addition, it was suggested that as a mechanism of tumor development, a variety of signaling pathways are activated by increased expression of cell proliferation-related factors.

  • Effects of excessive intake of trace elements and westernized high-fat diet on senile dementia

    System name: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C) (Commissioned Research)  Representative

    Project Year :

    2014.04
    -
    2017.03

    YOSHIDA KAORU

     View Summary

    Zinc (Zn) is an essential element but excess amount of Zn is known to cause neurotoxic effects. The risk of excessive intake of Zn is increased by intake from foods plus dietary supplements. We investigated the effects of chronic exposure to Zn on a step-through passive avoidance test (PA), a Y-maze test and a novel object recognition test (NOR) in aged mice. The behavioral tests indicated the possibilities that chronic exposure to Zn dose-dependently inhibited long-term memory and object recognition memory. The increased consumption of saturated fats in a westernized high-fat diet (HFD) contributes to neurodegenerative disease and senile dementia. We investigated whether HFD influences learning and memory deficits caused by chronic exposure to Zn in aged mice. The results of PA, NOR and a fear conditioning test showed that HFD feeding might result in an increase of object recognition memory and a decrease of long-term memory or context memory.

  • Inhibitory effects of carbonic anhydrase 2 inhibitor on the invasion of urinary bladder cancer

    System name: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C) (Commissioned Research)  Representative

    Project Year :

    2013.04
    -
    2016.03

    GI Min, TAMADA Satoshi

     View Summary

    We have demonstrated that that carbonic anhydrase 2 (CA2) is an invasion-associated factor in urothelial carcinoma (UC). The purpose to the present study is to examine the inhibitory effects of CA inhibitor Acetazolamide (Ace) on the N-butyl-N-(hydroxybutyl)nitrosameine (BBN)-induced mouse urinary bladder carcinogenesis. We found that concurrent treatment with Ace and Cisplatin (Cis) significantly inhibited the development of BBN-induced UC. Moreover, muscle invasive UC were significantly decreased by the Ace alone and concurrent treatment with Ace and Cis. These findings indicate that CA could serve as a potential therapeutic molecular target for bladder cancers and suggest that CA inhibitors are useful for prevention of UC invasion.

  • Investigation of Mechanism of Action of Steroid Sulfatase in Bladder Cancer

    System name: Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C) (Commissioned Research)  Representative

    Project Year :

    2012.04
    -
    2015.03

    TAMADA Satoshi, WEI Min, KATO Minoru

     View Summary

    we found out that steroid sulfatase (STS), an enzyme involved in androgen synthesis, is an important factor of invasion and prognosis of bladder cancer (BCa) patients. First, we demonstrated that the expression level of STS was significantly higher in muscle invasive BCa compared to non muscle-invasive BCa and the prognosis of STS positive Bca is significantly worse than that of STS negative BCa. Sedond, STS-knock down BCa cells showed less migration and invasion capability. Lastly, we showed that STS is relevant to epithelial to mesenchymal factors such as vimentin and ZEB1.
    In conclusion, we showed the possibility that STS is a novel target of BCa treatment.

display all >>