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Associate Professor

Laboratory location

Sugimoto Campus

Research Areas 【 display / non-display

Protein Engineering

Association Memberships 【 display / non-display

  • Protein Science Society of Japan

Current Career 【 display / non-display

  • Osaka City University   Graduate School of Engineering   Applied Chemistry and Bioengineering Course   Associate Professor  

Graduate School 【 display / non-display


    Osaka City University  Graduate School, Division of Engineering 

Graduating School 【 display / non-display


    Osaka City University   Faculty of Engineering  


Published Papers 【 display / non-display

  • Affinity maturation of humanized anti-epidermal growth factor receptor antibody using a modified phage-based open sandwich selection method.

    Sanada Hideaki, Kobayashi Kazuki, Oyama Kenji, Maru Takamitsu, Nakanishi Takeshi, Umetsu Mitsuo, Asano Ryutaro, Kumagai Izumi

    Scientific Reports  8   5414 2018.04  [Refereed]


  • Soluble expression in Escherichia coli of a single-domain antibody–tumor necrosis factor α fusion protein specific for epidermal growth factor receptor.

    Tomohiro Osaki, Takeshi Nakanishi, Motoshi Aoki, Takahiro Omizu, Daisuke Nishiura, Masaya Kitamura

    Monoclon. Antib. Immunodiagn. Immunother.  37 ( 1 ) 20 - 25 2018.02  [Refereed]

  • Photoinduced electron transfer from aromatic amino acids to the excited isoalloxazine in single mutated flavin mononucleotide binding proteins: Effect of the dimer formation on the rate and the electrostatic energy inside the proteins.

    Nadtanet Nunthaboot, Kiattisak Lugsanangarm, Arthit Nueangaudom, Somsak Pianwanit, Sirirat Kokpol, Fumio Tanaka, Seiji Taniguchi, Haik Chosrowjan, Takeshi Nakanishi, Masaya Kitamura

    Comput. Theor. Chem.  1108   1 - 9 2017  [Refereed]

  • Conformational difference between two subunits in flavin mononucleotide binding protein dimers from Desulfovibrio vulgaris (MF): Molecular dynamics simulation.

    N. Nunthaboot, K. Lugsanangarm, S. Pianwanit, S. Kokpol, F. Tanaka, T. Nakanishi, M. Kitamura

    Comput. Biol. Chem.  64   113 - 125 2016.10

  • Photoinduced electron transfer from aromatic amino acids to the excited isoalloxazine in flavin mononucleotide binding protein. Is the rate in the inverted region of donor – acceptor distance not real?

    N. Nunthaboot, K. Lugsanangarm, A. Pianwanit, F. Tanaka, S. Taniguchi, H. Chosrowjan, T. Nakanishi, M. Kitamura et al.

    J. Photochem. Photobiol. A: Chem.  326   60 - 68 2016.07

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Review Papers (Misc) 【 display / non-display

  • Protein-protein interactions and selection: generation of molecule-binding proteins on the basis of tertiary structural information

    FEBS J.  277 ( 9 ) 2006 - 2014 2010

  • Recent aspects of antibodylibrary technologies and their future perspectives

    26 ( 3 ) 254 - 257 2007

Grant-in-Aid for Scientific Research 【 display / non-display

  • Development of a molecular connection method for generating highly functional antibodies

    Project/Area Number : 25420836  Grant-in-Aid for Scientific Research(C) Representative

    Project Year :


     View Summary

    In this study, we used a bacterial expression system to produce a bispecific antibody that was heterotetramerized through hetero-associating peptides and that targeted cancer cells. As the results, we succeeded in preparing the recombinant antibody which bound to two target molecules simultaneously. Furthermore, the bispecific antibody showed cytotoxic activity against target tumor cells, and its cytotoxicity was comparable to that of a commercial therapeutic antibody. These results show that our method has high potential for the cost-efficient production of highly functional antibodies.

  • Development of a highly efficient method for generating functional antibodies based on grafting natural ligands

    Project/Area Number : 23790136  Grant-in-Aid for Young Scientists(B) Representative

    Project Year :


     View Summary

    In this study, to develop a method for generating functional antibodies, we grafted the peptide fragment, which was derived from the proteinous ligand, onto the variable region of a human antibody. As the results, we succeeded in preparing the peptide-gra