FUJITA Ken-ichi

写真a

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Title

Associate Professor

Laboratory location

Sugimoto Campus

Degree 【 display / non-display

  • Osaka City University -  Doctor of Science

Research Areas 【 display / non-display

Biopharmacology, Applied Microbiochemistry and Applied Biochemistry

Research Career 【 display / non-display

  • Screening and Mode of Action of Antifungal Antibiotics

    (Collaboration in Japan) Project Year :

    1998.04
    -
    Today

    Keyword in research subject:  Antifungal

  • Structure, biosynthetic mechanism and physiological activity of gamma-polylgutamic acid

    (Collaboration in Organization) Project Year :

    2005.04
    -
    Today

    Keyword in research subject:  high viscous polyamido

Association Memberships 【 display / non-display

  • THE SOCIETY FOR BIOTECHNOLOGY, JAPAN

Current Career 【 display / non-display

  • Osaka City University   Graduate School of Science   Biology and Geosciences Course   Associate Professor  

Career 【 display / non-display

  • 2003
     
     

    Osaka City University  

Graduate School 【 display / non-display

  •  
    -
    1996

    Osaka City University  Graduate School, Division of Natural Science 

Graduating School 【 display / non-display

  •  
    -
    1990

    Osaka City University   Faculty of Science  

 

Published Papers 【 display / non-display

  • Structure-activity relationships of antifungal phenylpropanoid derivatives and their synergy with n-dodecanol and fluconazole

    Y. Tsukuda, N. Mizuhara, Y. Usuki, Y. Yamaguchi, A. Ogita, T. Tanaka, K. Fujita

    Letters in Applied Microbiology  ( In press )  2021.11  [Refereed]

     View Summary

    trans-Anethole (anethole) is a phenylpropanoid; with other drugs, it exhibits synergistic activity against several fungi and is expected to be used in new therapies that cause fewer patient side effects. However, the detailed substructure(s) of the molecule responsible for this synergy has not been fully elucidated. We investigated the structure-activity relationships of phenylpropanoids and related derivatives, with particular attention on the methoxy group and the double bond of the propenyl group in anethole, as well as the length of the p-alkyl chain in p-alkylanisoles. Antifungal potency was largely related to p-alkyl chain length and the methoxy group of anethole, but not to the double bond of its propenyl group. Production of reactive oxygen species also played a role in these fungicidal activities. Inhibition of drug efflux was associated with the length of the p-alkyl chain and the double bond of the propenyl group in anethole, but not with the methoxy group. Although a desirable synergy was observed between n-dodecanol and anethole or p-alkylanisoles with a length of C2-C6 in alkyl chains, it cannot be explained away as being solely due to the inhibition of drug efflux. Similar results were obtained when phenylpropanoid derivatives were combined with fluconazole against Candida albicans.

    DOI

  • Involvement of a Multidrug Efflux Pump and Alterations in Cell Surface Structure in the Synergistic Antifungal Activity of Nagilactone E and Anethole against Budding Yeast Saccharomyces cerevisiae

    Yuki Ueda, Yuhei O. Tahara, Makoto Miyata, Akira Ogita, Yoshihiro Yamaguchi, Toshio Tanaka and Ken-ichi Fujita

    Antibiotics  10 ( 5 ) 537 2021.05  [Refereed]  [Invited]

    DOI

  • Enhancing the fungicidal activity of amphotericin B via vacuole disruption by benzyl isothiocyanate, a cruciferous plant constituent

    N. Yamada, W. Murata, Y. Yamaguchi, K.-I. Fujita, A. Ogita, T. Tanaka

    Letters in Applied microbiology  72 ( 4 ) 390 - 398 2021.04  [Refereed]

  • Effect of pgsE expression on the molecular weight of poly(γ-glutamic acid) in fermentative production

    Ken-Ichi Fujita, Takashi Tomiyama, Takahiro Inoi, Takashi Nishiyama, Eriko Sato, Hideo Horibe, Ryosuke Takahashi, Shinichi Kitamura, Yoshihiro Yamaguchi, Akira Ogita and Toshio Tanaka

    Polymer Journal  53 ( 2 ) 409 - 414 2021.02  [Refereed]

  • Evaluation of fatty acid synthase as a molecular target for stress-dependent fungicidal activity of 1-geranylgeranylpyridinium

    Doi T, Miyuki S, Usuki Y, Yamaguchi Y, Fujita K, OgitaA , Tanaka T

    Global Journal of Medical Research  20 ( 2 ) 1 - 9 2020.03  [Refereed]

     View Summary

    さまざまなイソプレノイドとその誘導体の中で、1-geranylgeranylpyridinium(GGPy)は浸透ストレス依存性の致死性に加えて温度依存性の致死効果を示した。脂肪酸シンターゼ(FAS)は、GGPy結合タンパク質の1つとして同定され、真菌のストレス適応に対する阻害効果においてGGPyの分子標的とみなされた。これらの成果は、上記科目で主題として扱う生理活性物質およびその作用機構に該当する。

    DOI

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Books etc 【 display / non-display

  • Anti-Salmonella agents from the Brazilian medicinal plant Tanacetum balsamita and their applications

    Kubo I, Fujita K, Shimizu K. (Part: Contributor )

    Natural Antioxidants and Biocides from Wild Medicinal Plants (eds C.L. Cespedes et al.) CAB International  2013

     View Summary

    ブラジルのコショウ農場で養鶏場由来のサルモネラ汚染が問題となっている。本稿では、ブラジルの薬用植物 Tanacetum balsamita に含まれる抗サルモネラ活性成分について概説するとともに、最も効果的であったトランス・ヘキセナールについてその応用性を概説した。

  • Naturally Occurring antifungal agents and their modes of action

    Kubo I, Shimizu K, Fujita K. (Part: Joint Work )

    Fungicides for Plant and Animal Diseases. Edited by: D. Dhanasekaran, N. Thajuddin and A. Panneerselvam Publisher: InTech  2012

     View Summary

    免疫抑制剤や抗がん剤の投与に加えて、加齢による免疫力が低下したヒトが増加しており、真菌の日和見感染が問題となってきている。本稿では、臨床で用いられる抗真菌性抗生物質と比較しながら、植物由来天然物の抗真菌活性の特徴(抗生物質に比べて概して活性は弱い)およびその作用機序(細胞膜障害作用を示す物質が多い)について概説した。

Review Papers (Misc) 【 display / non-display

  • Molecular design of multifunctional anti-Salmonella agents based on natural products

    Kubo I, Fujita K, Nihei K, Kubo A.

    Advances in Phytomedicine  2   353 - 372 2006

     View Summary

    ブラジルのコショウ農場で養鶏場由来のサルモネラ汚染が問題となっている。本稿では、植物由来天然成分に含まれる抗サルモネラ活性の特徴やそれらの作用機序について概説した。抗サルモネラ成分として、直鎖のアルコールやアルデヒドに加えてトランス・アルケナール類を見いだした。それらのほとんどは細胞膜障害作用を示したが、アルデヒドやトランス・アルケナール類はその反応性の高さから細胞質の様々な成分と反応する可能性が指摘された。

  • Molecular design of antioxidative anti-Salmonella agents

    Kubo I, Fujita K, Nihei K

    Revista Latinoamericana de Quimica  33 ( 2 ) 60 - 75 2005  [Refereed]  [Invited]

     View Summary

    ブラジルのコショウ農場でサルモネラの汚染が問題となっている。抗サルモネラ剤を抗酸化作用を示す没食子酸エステルを中心にデザインした。すなわち、抗サルモネラ活性を最適化するためにそのエステル類のアルキル鎖長を変化させた。その結果、炭素数8および9の没食子酸エステルは最も効果的であった。またそれらのエステルは膜障害作用を示した。

Conference Activities & Talks 【 display / non-display

  • Structure-activity relationships in antifungal activities of phenylpropanoid derivatives including trans-anethole and their synergy in combination with n-dodecanol

    Y. Tsukuda,N. Mizuhara,Y. Usuki,W. Murata,Y. Yamaguchi,A. Ogita,T. Tanaka,K. Fujita

    The 14th World Congress on Polyphenol Applications  (オンライン)  2021.09 

  • Anti-Aging Effects of Hydrophobic and Hydrophilic Components From Immature Pear Fruits Extract

    Ogita A, Murata W, Sakai A, Yamaguchi Y, Tanaka T, Fujita K

    GSA 2020 Annual Scientific Meeting ONLINE  2020.11 

  • Prolongation of Human Lifespan by Immature Pear Extract Mediated Sirtuin-Related Gene Expression

    Akira Ogita, Wakae Murata, Marina Hasegawa, Ken Yamauchi, Akiko Sakai, Yoshihiro Yamaguchi, Toshio Tanaka, Ken-ichi Fujita

    The Gerontological Society of America (GSA) 2019 Annual Scientific Meeting  (Austin, Texas)  2019.11 

  • Synergistic antifungal mechanism induced by nagilactone E in combinationwith anethole

    2019.09 

  • Effects of water and ethanol soluble components from unripe pear fruit onlifespan extension and gene expression of budding yeast

    2019.09 

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Other educational activity and Special note 【 display / non-display

  • Office hours

    (2021)

  • Office hours

    (2020)

  • Office hours

    (2019)

  • Office hours

    (2018)

  • Office hours

    (2017)

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Foreigner acceptance 【 display / non-display

  • Academic year : 2018

     View Details

    Number of foreigners accepted
    0
  • Academic year : 2017

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    Number of foreigners accepted
    0