FUJITA Ken-ichi


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Associate Professor

Laboratory location

Sugimoto Campus

Degree 【 display / non-display

  • Osaka City University -  Doctor of Science

Research Areas 【 display / non-display

Biopharmacology, Applied Microbiochemistry and Applied Biochemistry

Research Career 【 display / non-display

  • Screening and Mode of Action of Antifungal Antibiotics

    (Collaboration in Japan) Project Year :


    Keyword in research subject:  Antifungal

  • Structure, biosynthetic mechanism and physiological activity of gamma-polylgutamic acid

    (Collaboration in Organization) Project Year :


    Keyword in research subject:  high viscous polyamido

Association Memberships 【 display / non-display


Current Career 【 display / non-display

  • Osaka City University   Graduate School of Science   Biology and Geosciences Course   Associate Professor  

Career 【 display / non-display

  • 2003

    Osaka City University  

Graduate School 【 display / non-display


    Osaka City University  Graduate School, Division of Natural Science 

Graduating School 【 display / non-display


    Osaka City University   Faculty of Science  


Published Papers 【 display / non-display

  • Structure-activity relationships of antifungal phenylpropanoid derivatives and their synergy with n-dodecanol and fluconazole

    Y. Tsukuda, N. Mizuhara, Y. Usuki, Y. Yamaguchi, A. Ogita, T. Tanaka, K. Fujita

    Letters in Applied Microbiology  ( In press )  2021.11  [Refereed]

     View Summary

    trans-Anethole (anethole) is a phenylpropanoid; with other drugs, it exhibits synergistic activity against several fungi and is expected to be used in new therapies that cause fewer patient side effects. However, the detailed substructure(s) of the molecule responsible for this synergy has not been fully elucidated. We investigated the structure-activity relationships of phenylpropanoids and related derivatives, with particular attention on the methoxy group and the double bond of the propenyl group in anethole, as well as the length of the p-alkyl chain in p-alkylanisoles. Antifungal potency was largely related to p-alkyl chain length and the methoxy group of anethole, but not to the double bond of its propenyl group. Production of reactive oxygen species also played a role in these fungicidal activities. Inhibition of drug efflux was associated with the length of the p-alkyl chain and the double bond of the propenyl group in anethole, but not with the methoxy group. Although a desirable synergy was observed between n-dodecanol and anethole or p-alkylanisoles with a length of C2-C6 in alkyl chains, it cannot be explained away as being solely due to the inhibition of drug efflux. Similar results were obtained when phenylpropanoid derivatives were combined with fluconazole against Candida albicans.


  • Involvement of a Multidrug Efflux Pump and Alterations in Cell Surface Structure in the Synergistic Antifungal Activity of Nagilactone E and Anethole against Budding Yeast Saccharomyces cerevisiae

    Yuki Ueda, Yuhei O. Tahara, Makoto Miyata, Akira Ogita, Yoshihiro Yamaguchi, Toshio Tanaka and Ken-ichi Fujita

    Antibiotics  10 ( 5 ) 537 2021.05  [Refereed]  [Invited]


  • Enhancing the fungicidal activity of amphotericin B via vacuole disruption by benzyl isothiocyanate, a cruciferous plant constituent

    N. Yamada, W. Murata, Y. Yamaguchi, K.-I. Fujita, A. Ogita, T. Tanaka

    Letters in Applied microbiology  72 ( 4 ) 390 - 398 2021.04  [Refereed]

  • Effect of pgsE expression on the molecular weight of poly(γ-glutamic acid) in fermentative production

    Ken-Ichi Fujita, Takashi Tomiyama, Takahiro Inoi, Takashi Nishiyama, Eriko Sato, Hideo Horibe, Ryosuke Takahashi, Shinichi Kitamura, Yoshihiro Yamaguchi, Akira Ogita and Toshio Tanaka

    Polymer Journal  53 ( 2 ) 409 - 414 2021.02  [Refereed]

  • Evaluation of fatty acid synthase as a molecular target for stress-dependent fungicidal activity of 1-geranylgeranylpyridinium

    Doi T, Miyuki S, Usuki Y, Yamaguchi Y, Fujita K, OgitaA , Tanaka T

    Global Journal of Medical Research  20 ( 2 ) 1 - 9 2020.03  [Refereed]

     View Summary



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Books etc 【 display / non-display

  • Anti-Salmonella agents from the Brazilian medicinal plant Tanacetum balsamita and their applications

    Kubo I, Fujita K, Shimizu K. (Part: Contributor )

    Natural Antioxidants and Biocides from Wild Medicinal Plants (eds C.L. Cespedes et al.) CAB International  2013

     View Summary

    ブラジルのコショウ農場で養鶏場由来のサルモネラ汚染が問題となっている。本稿では、ブラジルの薬用植物 Tanacetum balsamita に含まれる抗サルモネラ活性成分について概説するとともに、最も効果的であったトランス・ヘキセナールについてその応用性を概説した。

  • Naturally Occurring antifungal agents and their modes of action

    Kubo I, Shimizu K, Fujita K. (Part: Joint Work )

    Fungicides for Plant and Animal Diseases. Edited by: D. Dhanasekaran, N. Thajuddin and A. Panneerselvam Publisher: InTech  2012

     View Summary


Review Papers (Misc) 【 display / non-display

  • Molecular design of multifunctional anti-Salmonella agents based on natural products

    Kubo I, Fujita K, Nihei K, Kubo A.

    Advances in Phytomedicine  2   353 - 372 2006

     View Summary


  • Molecular design of antioxidative anti-Salmonella agents

    Kubo I, Fujita K, Nihei K

    Revista Latinoamericana de Quimica  33 ( 2 ) 60 - 75 2005  [Refereed]  [Invited]

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Conference Activities & Talks 【 display / non-display

  • Structure-activity relationships in antifungal activities of phenylpropanoid derivatives including trans-anethole and their synergy in combination with n-dodecanol

    Y. Tsukuda,N. Mizuhara,Y. Usuki,W. Murata,Y. Yamaguchi,A. Ogita,T. Tanaka,K. Fujita

    The 14th World Congress on Polyphenol Applications  (オンライン)  2021.09 

  • Anti-Aging Effects of Hydrophobic and Hydrophilic Components From Immature Pear Fruits Extract

    Ogita A, Murata W, Sakai A, Yamaguchi Y, Tanaka T, Fujita K

    GSA 2020 Annual Scientific Meeting ONLINE  2020.11 

  • Prolongation of Human Lifespan by Immature Pear Extract Mediated Sirtuin-Related Gene Expression

    Akira Ogita, Wakae Murata, Marina Hasegawa, Ken Yamauchi, Akiko Sakai, Yoshihiro Yamaguchi, Toshio Tanaka, Ken-ichi Fujita

    The Gerontological Society of America (GSA) 2019 Annual Scientific Meeting  (Austin, Texas)  2019.11 

  • Synergistic antifungal mechanism induced by nagilactone E in combinationwith anethole


  • Effects of water and ethanol soluble components from unripe pear fruit onlifespan extension and gene expression of budding yeast


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Other educational activity and Special note 【 display / non-display

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Foreigner acceptance 【 display / non-display

  • Academic year : 2018

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    Number of foreigners accepted
  • Academic year : 2017

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    Number of foreigners accepted