MIYATA Makoto

写真a

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Title

Professor

Laboratory location

Sugimoto Campus

Homepage URL

http://www.sci.osaka-cu.ac.jp/~miyata/

Mail Address

E-mail address

Degree 【 display / non-display

  • Osaka University -  Doctor of Science

Research Areas 【 display / non-display

Biophysics, Bacteriology

Research subject summary 【 display / non-display

  • A bacterial Class, Mollicutes evolved from Phylum Firmicutes to be more specialized to their parasitic life style. They quit peptidoglycan synthesis probably to evade natural immune system of host, resulting in loss of bacterial conventional motility systems such as flagella and pili. Instead, they developed at least three independent motility systems, including two surface motilities.

    Mycoplasma mobile, a fish pathogen form a gliding machinery at a pole and glide on solid surfaces with a speed up to 4.5 μm/s, to the direction of the machinery. We have been studying about structures and components of gliding machinery, binding target, energy source, mechanical characters, and then proposed a working model, "Force generated by a special motor originated from ATP synthase transmits across cell membrane through several proteins, resulting in catch, pull, and release with the sialylated oligosaccharides on host surfaces."

    Since 2012, we are developing the Quick-Freeze Deep-Etch Replica Electron Microscopy method that is useful for studying the motility mechanism, and we are providing this technology to collaborators all over the world.

Research Interests 【 display / non-display

Motility evolution, Mycoplasma gliding, Spiroplasma swimming, Class mollicutes, Molecular mechanism, Quick freeze replica electron microscopy, Motility machinery

Research Career 【 display / non-display

  • Development of Quick-Freeze Deep-Etch Replica Electron Microscopy method useful for studying motion mechanism

    (Individual) Project Year :

    2012
    -
    Today

  • Mechanism of Spiroplasma swimming movement

    (Individual) Project Year :

    2011
    -
    Today

  • Molecular Mechanism of Gliding Motility of Mycoplasma

    (Individual) Project Year :

    1997
    -
    Today

  • Mycoplasma DNA replication and cell division

    (Individual) Project Year :

    1988
    -
    1997

  • Biochemical study of muscle myosin

    (Individual) Project Year :

    1982
    -
    1988

Association Memberships 【 display / non-display

  • The Biophysical Society of Japan

  • American Society for Microbiology

  • International Organization for Mycoplasmology

  • Japanese Society for mycoplasmology

  • Japanese Society of Bacteriology

Committee Memberships 【 display / non-display

  • 2021.04
    -
    Today

    The Biophysical Society of Japan   The Biophysics and Physicobiology (BPPB) Editorial Board

  • 2018.12
    -
    2022.07

    International Organization for Mycoplasmology   Chairperson of IOM's annual meeting in 2022

  • 2018.06
    -
    2020.12

    The Biophysical Society of Japan   Chair of website committee

  • 2018.04
    -
    2019.12

    The Biophysical Society of Japan   Chair of executive committee of annual meeting 2019

  • 2017.06
    -
    2020.03

    The Biophysical Society of Japan   Board

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Awards & Honors 【 display / non-display

  • Presentation award at the 59th annual meeting of the Biophysical society of Japan

    ATP hydrolysis mechanism of bacterial actin MreB

    2021.11.27   Biophysical society of Japan

    Winner : D2 Daichi Takahashi

  • The 36th Best paper award

    Peptidoglycan layer and disruption processes in Bacillus subtilis cells visualized using quick-freeze, deep-etch electron microscopy

    2021.06.15   Japanese Society of Microscopy

    Winner : Researcher Yuhei Tahara

  • Presentation award at the 94th annual meeting of Japanese Society of Bacteriology

    Spiroplasma swimming motility to synthetic bacterium JCVI-syn3.0

    2021.03.25   Japanese Society of Bacteriology

    Winner : M2 Hana Kiyama

  • Presentation award at the 94th annual meeting of Japanese Society of Bacteriology

    Sructural basis of bacterial actin MreB involved in Spiroplasma swimming

    2021.03.25   Japanese Society of Bacteriology

    Winner : D1 Daichi Takahashi

  • Faculty Activity Awardee

    2020.11.25   Osaka City University

    Winner : Makoto Miyata

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Current Career 【 display / non-display

  • Osaka City University   Graduate School of Science   Biology and Geosciences Course   Professor  

Career 【 display / non-display

  • 2019.10
    -
    2025.03

    Strategic basic research program (CREST) by JST, "Large-scale genome synthesis and cell programming"   Research director

  • 2012.04
    -
    2017.03

    Grand-in-Aid "Harmonized supramolecular motility machinery and its diversity"   Leader

  • 2006.10
    -
    Today

    Osaka City University   Graduate school of Science, Biology & Geosciences,   Professor

  • 2003.10
    -
    2007.03

    PRESTO JST   researcher

  • 2000.03
    -
    2001.03

    Rowland Institute for Science and Harvard University   Visiting Scholar

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Graduate School 【 display / non-display

  • 1983.04
    -
    1988.03

    Osaka University  Graduate School, Division of Natural Science  physiology 

Graduating School 【 display / non-display

  • 1979.04
    -
    1983.03

    Osaka University   Faculty of Science   Department of Biology

 

Published Papers 【 display / non-display

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Books etc 【 display / non-display

  • Gliding mechanism of Mycoplasma pneumoniae subgroup -implication from Mycoplasma mobile-.

    Miyata M and Nakane D (Part: Joint Work )

    Mollicutes: Molecular Biology and Pathogenesis. Browning G, Citti C (Ed.) Caister Academic Press.  2013

  • Molecular mechanism of mycoplasma gliding - a novel cell motility system.

    Jacques Prost, Kinneret Keren, Herbert Levine, Marileen Dogterom, Sarah Rice, Makoto Miyata, Tanniemola B. Liverpool, Peter Lenz (Part: Joint Work )

    Cell Motility, Lenz P (Ed.), Springer. NewYork  2007.11

     View Summary

    Cell motility is a fascinating example of cell behavior which is fundamentally important to a number of biological and pathological processes. It is based on a complex self-organized mechano-chemical machine consisting of cytoskeletal filaments and molecular motors. In general, the cytoskeleton is responsible for the movement of the entire cell and for movements within the cell. The main challenge in the field of cell motility is to develop a complete physical description on how and why cells move. For this purpose new ways of modeling the properties of biological cells have to be found. This long term goal can only be achieved if new experimental techniques are developed to extract physical information from these living systems and if theoretical models are found which bridge the gap between molecular and mesoscopic length scales. Cell Motility gives an authoritative overview of the fundamental biological facts, theoretical models, and current experimental developments in this fascinating area.

  • Gliding motility of mycoplasmas -A mechanism cannot be explained by current biology-

    Miyata M (Part: Joint Work )

    Mycoplasmas: molecular biology, pathogenecity and strategies for control. Blanchard A, Browning G (Ed.), CRC Press.  2005.04

  • Cell division

    Miyata M (Part: Joint Work )

    Molecular Biology and Pathogenicity of Mycoplasmas. Herrmann R, Razin S (Ed.) Springer, Boston, MA  2002

Review Papers (Misc) 【 display / non-display

Conference Activities & Talks 【 display / non-display

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Grant-in-Aid for Scientific Research 【 display / non-display

  • Three types of motility mechanisms in pathogenic bacteria, class Mollicutes

    Project/Area Number : 17H01544  Grant-in-Aid for Scientific Research(A) Representative

    Project Year :

    2017.04
    -
    2020.03
     

  • Generalization of harminized supermolecular motility machinery and its diversity

    Project/Area Number : 17H06082  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area) Representative

    Project Year :

    2017.04
    -
    2018.03
     

  • Dynamic bacterial cytoskeleton in Spiroplasma

    Project/Area Number : 15H03712  Grant-in-Aid for Scientific Research(B) Representative

    Project Year :

    2015.04
    -
    2018.03
     

    Partaker : MIYATA Makoto, NAKANE Daisuke

     View Summary

    We have revealed some new aspects on the mechanics of unique swimming motility of bacterium Spiroplasma. In particular, we have constructed the minimal mechanistic model that may explain helical morphologies of Spiroplasma based on the structural biological data on the ribbon-like cytoskeleton. We have also determined the elastic rigidities of Spiroplasma cell by combining theoretical and numerical analysis with the data from the micromanipulation experiments. In addition to these, we have studied fundamental geometric mechanical properties of macro-scale elastic ribbons by combining theory and experiment, and discussed our new findings in relation to cellular mechanics of bacteria. To summarize, our achievement during the period of this project will significantly contribute to the understanding of yet mysterious mechanism of Spiroplasma swimming dynamics.

  • Mechanism of Mycoplasma gliding

    Project/Area Number : 24117002  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area) Representative

    Project Year :

    2012.06
    -
    2017.03
     

  • Harmonized supramolecular machinery for motility and its diversity

    Project/Area Number : 3407  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area) Representative

    Project Year :

    2012.06
    -
    2017.03
     

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Other external funds procured 【 display / non-display

  • Construction of cell evolution model using synthetic bacterium JCVI syn3.0B and genome manipulation

    System name: Strategic Basic Research Programs (Subsidy)  Representative

    Project Year :

    2019.10
    -
    2025.03

 

Charge of on-campus class subject 【 display / non-display

  • Current Biology

    (2021) University, Special course

  • Today's Natural Sciences and Human Beings

    (2021) University, General education courses

  • Biological Science

    (2021) University, Special course

  • Advanced Laboratory in Biology A

    (2021) University, Special course

  • Biological Laboratory B

    (2021) University, General education courses

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Educational activity record 【 display / non-display

  • Academic year : 2021

     View Details

    Number of instructed the graduation thesis
    1
    Number of graduation thesis reviews
    0
    Number of instructed the Master course
    1
    Number of instructed the Doctoral course
    5
    Number of master's thesis reviews (chief)
    1
    Number of master's thesis reviews (vice-chief)
    8
    Number of doctoral thesis reviews (chief)
    1
    Number of doctoral thesis reviews (vice-chief)
    0
  • Academic year : 2020

     View Details

    Number of instructed the graduation thesis
    2
    Number of graduation thesis reviews
    1
    Number of instructed the Master course
    3
    Number of instructed the Doctoral course
    4
    Number of master's thesis reviews (chief)
    2
    Number of master's thesis reviews (vice-chief)
    4
    Number of doctoral thesis reviews (chief)
    0
    Number of doctoral thesis reviews (vice-chief)
    1
  • Academic year : 2019

     View Details

    Number of instructed the graduation thesis
    4
    Number of graduation thesis reviews
    0
    Number of instructed the Master course
    5
    Number of instructed the Doctoral course
    4
    Number of master's thesis reviews (chief)
    3
    Number of master's thesis reviews (vice-chief)
    4
    Number of doctoral thesis reviews (chief)
    1
    Number of doctoral thesis reviews (vice-chief)
    0
  • Academic year : 2018

     View Details

    Number of instructed the graduation thesis
    2
    Number of graduation thesis reviews
    0
    Number of instructed the Master course
    3
    Number of instructed the Doctoral course
    4
    Number of master's thesis reviews (chief)
    0
    Number of master's thesis reviews (vice-chief)
    5
    Number of doctoral thesis reviews (chief)
    0
    Number of doctoral thesis reviews (vice-chief)
    0
  • Academic year : 2017

     View Details

    Number of instructed the graduation thesis
    3
    Number of graduation thesis reviews
    0
    Number of instructed the Master course
    2
    Number of instructed the Doctoral course
    4
    Number of master's thesis reviews (chief)
    2
    Number of master's thesis reviews (vice-chief)
    6
    Number of doctoral thesis reviews (chief)
    0
    Number of doctoral thesis reviews (vice-chief)
    0

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Theme of Possible Research Exchange 【 display / non-display

  • Molecular mechanism of adhesion and gliding in mycoplasmas

    Request for collaborative research : The private sector, such as other institutions

    Type of research exchange : Technical consultation, Consignment study, Collaborative research, Lecture

    Keyword : nanoacutuator, infetious disease, mycoplasma, binding, protein

 

Foreigner acceptance 【 display / non-display

  • Academic year : 2021

     View Details

    Number of foreigners accepted
    0
    Number of International Students
    2
  • Academic year : 2020

     View Details

    Number of foreigners accepted
    1
    Number of International Students
    1

    イギリス

  • Academic year : 2019

     View Details

    Number of foreigners accepted
    1
    Number of International Students
    1

    イギリス

  • Academic year : 2018

     View Details

    Number of foreigners accepted
    1
    Number of International Students
    1

    フランス

  • Academic year : 2017

     View Details

    Number of foreigners accepted
    3
    Number of International Students
    2

    トルコ、アメリカ合衆国(2名)

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