MIYATA Makoto

写真a

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Title

Professor

Laboratory location

Sugimoto Campus

Homepage URL

http://www.sci.osaka-cu.ac.jp/~miyata/myco1.htm

Mail Address

E-mail address

Degree 【 display / non-display

  • Osaka University -  Doctor of Science

Research Areas 【 display / non-display

Biophysics, Bacteriology

Research subject summary 【 display / non-display

  • A bacterial Class, Mollicutes evolved from Phylum Firmicutes to be more specialized to their parasitic life style. They quit peptidoglycan synthesis probably to evade natural immune system of host, resulting in loss of bacterial conventional motility systems such as flagella and pili. Instead, they developed at least three independent motility systems, including two surface motilities.

    Mycoplasma mobile, a fish pathogen form a gliding machinery at a pole and glide on solid surfaces with a speed up to 4.5 μm/s, to the direction of the machinery. We have been studying about structures and components of gliding machinery, binding target, energy source, mechanical characters, and then proposed a working model, "Force generated by a special motor originated from ATP synthase transmits across cell membrane through several proteins, resulting in catch, pull, and release with the sialylated oligosaccharides on host surfaces."

    Since 2012, we are developing the Quick-Freeze Deep-Etch Replica Electron Microscopy method that is useful for studying the motility mechanism, and we are providing this technology to collaborators all over the world.

Research Interests 【 display / non-display

spiroplasma, Protein, mycoplasma, Class mollicutes, Quick freeze replica electron microscopy, Gliding motility, Swimming motility, Motility machinery, Gene

Research Career 【 display / non-display

  • Development of Quick-Freeze Deep-Etch Replica Electron Microscopy method useful for studying motion mechanism

    (Individual) Project Year :

    2012
    -
    Today

  • Mechanism of Spiroplasma swimming movement

    (Individual) Project Year :

    2011
    -
    Today

  • Molecular Mechanism of Gliding Motility of Mycoplasma

    (Individual) Project Year :

    1997
    -
    Today

  • Mycoplasma DNA replication and cell division

    (Individual) Project Year :

    1988
    -
    1997

  • Biochemical study of muscle myosin

    (Individual) Project Year :

    1982
    -
    1988

Association Memberships 【 display / non-display

  • The Biophysical Society of Japan

  • American Society for Microbiology

  • International Organization for Mycoplasmology

  • Japanese Society for mycoplasmology

  • Japanese Society of Bacteriology

Committee Memberships 【 display / non-display

  • 2018.12
    -
    2022.07

    International Organization for Mycoplasmology   Chairperson of IOM's annual meeting in 2022

  • 2018.06
    -
    2020.12

    The Biophysical Society of Japan   Chair of website committee

  • 2018.04
    -
    2019.12

    The Biophysical Society of Japan   Chair of executive committee of annual meeting 2019

  • 2017.06
    -
    Today

    The Biophysical Society of Japan   Board

  • 2017.01
    -
    Today

    Scientific Reports   editorial board member

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Awards & Honors 【 display / non-display

  • President's award at the 10th OCARINA International Symposium

    Reproduction of spiroplasma swimming motility using synthetic bacteria and elucidation of its mechanism

    2019.03.05   The OCU Advanced Research Institute for Natural Science and Technology

    Winner : B4 Hana Kiyama

  • Poster award at the 10th OCARINA International Symposium

    Structure of motor evolved by combination of ATP synthase and phosphoglycerate kinase for Mycoplasma mobile gliding

    2019.03.05   The OCU Advanced Research Institute for Natural Science and Technology

    Winner : D2 Takuma Toyonaga

  • Poster award at the 10th OCARINA International Symposium

    Identification and characterization of genes involved in constructing spike structure of fission yeast spore surface

    2019.03.05   The OCU Advanced Research Institute for Natural Science and Technology

    Winner : B4 Daiki Masuda (Prof Taro Nakamura, Osaka City Univ, Collaboration)

  • The theses presentation award of Biology Department

    Reproduction of Spiroplasma eriocheiris swimming motility using synthetic bacteria

    2019.03.05   Osaka City University, Faculty of Science

    Winner : B4 Hana Kiyama

  • Poster award at the Membrane Symposium 2018

    Mechanism analysis of multilamellar vesicle secretion by microorganisms

    2018.11.14   the Membrane society of Japan

    Winner : Mr Kotaro Takagi (Dr. Yosuke Tashiro, Shizuoka Univ. Collaboration)

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Current Career 【 display / non-display

  • Osaka City University   Graduate School of Science   Biology and Geosciences Course   Professor  

Career 【 display / non-display

  • 2012.04
    -
    2017.03

    Grand-in-Aid "Harmonized supramolecular motility machinery and its diversity"   Leader

  • 2006.10
    -
    Today

    Osaka City University   Graduate school of Science, Biology & Geosciences,   Professor

  • 2003.10
    -
    2007.03

    PRESTO JST   researcher

  • 2000.03
    -
    2001.03

    Rowland Institute for Science and Harvard University   Visiting Scholar

  • 1998.10
    -
    2006.09

    Osaka City University   Faculty of Science, Department of Biology   assistant professor

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Graduate School 【 display / non-display

  • 1983.04
    -
    1988.03

    Osaka University  Graduate School, Division of Natural Science  physiology 

Graduating School 【 display / non-display

  • 1979.04
    -
    1983.03

    Osaka University   Faculty of Science   Department of Biology

 

Published Papers 【 display / non-display

  • Production and characterization of recombinant P1 adhesin essential for adhesion, gliding, and antigenic variation in the human pathogenic bacterium, Mycoplasma pneumoniae

    Kenri T, Kawakita Y, Kudo H, Matsumoto U, Mori S, Furukawa Y, Tahara YO, Shibayama K, Hayashi Y, Arai M, Miyata M

    Biochemical and Biophysical Research Communications  508 ( 4 ) 1050 - 1055 2019.01  [Refereed]

    DOI PubMed

  • Identification of novel protein domain for sialyloligosaccharide binding essential to Mycoplasma mobile gliding.

    Hamaguchi T, Kawakami M, Furukawa H, Miyata M

    FEMS Microbiology Letters  2019.01  [Refereed]

    DOI

  • Linear motor driven-rotary motion of a membrane-permeabilized ghost in Mycoplasma mobile.

    Kinosita Y, Miyata M, Nishizaka T

    Scientific Reports  8 ( 1 ) 11513 2018.07  [Refereed]

    DOI PubMed

  • Detailed analyses of stall force generation in Mycoplasma mobile gliding

    Mizutani M, Tulum I, Kinosita Y, Nishizaka T, Miyata M

    Biophysical Journal  114 ( 6 ) 1411 - 1419 2018.03  [Refereed]

    DOI

  • Transformation of crustacean pathogenic bacterium Spiroplasma eriocheiris and expression of yellow fluorescent protein

    Terahara N, Tulum I, and Miyata M

    Biochemical and Biophysical Research Communications   487 ( 3 ) 488 - 493 2017.06  [Refereed]

    DOI

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Books etc 【 display / non-display

  • Gliding mechanism of Mycoplasma pneumoniae subgroup -implication from Mycoplasma mobile-.

    Miyata M and Nakane D (Part: Joint Work )

    Mollicutes: Molecular Biology and Pathogenesis. Browning G, Citti C (Ed.) Caister Academic Press.  2013

  • Molecular mechanism of mycoplasma gliding - a novel cell motility system.

    Jacques Prost, Kinneret Keren, Herbert Levine, Marileen Dogterom, Sarah Rice, Makoto Miyata, Tanniemola B. Liverpool, Peter Lenz (Part: Joint Work )

    Cell Motility, Lenz P (Ed.), Springer. NewYork  2007.11

     View Summary

    Cell motility is a fascinating example of cell behavior which is fundamentally important to a number of biological and pathological processes. It is based on a complex self-organized mechano-chemical machine consisting of cytoskeletal filaments and molecular motors. In general, the cytoskeleton is responsible for the movement of the entire cell and for movements within the cell. The main challenge in the field of cell motility is to develop a complete physical description on how and why cells move. For this purpose new ways of modeling the properties of biological cells have to be found. This long term goal can only be achieved if new experimental techniques are developed to extract physical information from these living systems and if theoretical models are found which bridge the gap between molecular and mesoscopic length scales. Cell Motility gives an authoritative overview of the fundamental biological facts, theoretical models, and current experimental developments in this fascinating area.

  • Gliding motility of mycoplasmas -A mechanism cannot be explained by current biology-

    Miyata M (Part: Joint Work )

    Mycoplasmas: molecular biology, pathogenecity and strategies for control. Blanchard A, Browning G (Ed.), CRC Press.  2005.04

  • Cell division

    Miyata M (Part: Joint Work )

    Molecular Biology and Pathogenicity of Mycoplasmas. Herrmann R, Razin S (Ed.) Springer, Boston, MA  2002

Review Papers (Misc) 【 display / non-display

Conference Activities & Talks 【 display / non-display

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Grant-in-Aid for Scientific Research 【 display / non-display

  • Three types of motility mechanisms in pathogenic bacteria, class Mollicutes

    Project/Area Number : 17H01544  Grant-in-Aid for Scientific Research(A) Representative

    Project Year :

    2017.04
    -
    2020.03
     

  • Generalization of harminized supermolecular motility machinery and its diversity

    Project/Area Number : 17H06082  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area) Representative

    Project Year :

    2017.04
    -
    2018.03
     

  • Harmonized supramolecular machinery for motility and its diversity

    Project/Area Number : 3407  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area) Representative

    Project Year :

    2012.06
    -
    2017.03
     

  • Generalization of harmonized supramolecular motility machinery and its diversity

    Project/Area Number : 24117001  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area) Representative

    Project Year :

    2012.06
    -
    2017.03
     

  • Mechanism of Mycoplasma gliding

    Project/Area Number : 24117002  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area) Representative

    Project Year :

    2012.06
    -
    2017.03
     

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Charge of on-campus class subject 【 display / non-display

  • Science Basic Seminar

    (2018) University, Special course

  • Current Biology

    (2018) University, Special course

  • Advanced Seminar in Biology

    (2018) University, Special course

  • Biological Laboratory B

    (2018) University, General education courses

  • Seminar in Sciences of Biomolecules

    (2018) Graduate school, Special course

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Educational activity record 【 display / non-display

  • Academic year : 2018

     View Details

    Number of instructed the graduation thesis
    2
    Number of graduation thesis reviews
    0
    Number of instructed the Master course
    3
    Number of instructed the Doctoral course
    4
    Number of master's thesis reviews (chief)
    0
    Number of master's thesis reviews (vice-chief)
    5
    Number of doctoral thesis reviews (chief)
    0
    Number of doctoral thesis reviews (vice-chief)
    0
  • Academic year : 2017

     View Details

    Number of instructed the graduation thesis
    3
    Number of instructed the Master course
    2
    Number of instructed the Doctoral course
    4
    Number of master's thesis reviews (chief)
    2
    Number of master's thesis reviews (vice-chief)
    6
    Number of doctoral thesis reviews (chief)
    0
    Number of doctoral thesis reviews (vice-chief)
    0
  • Academic year : 2016

     View Details

    Number of instructed the graduation thesis
    3
    Number of instructed the Master course
    7
    Number of instructed the Doctoral course
    0
    Number of master's thesis reviews (chief)
    5
    Number of master's thesis reviews (vice-chief)
    4
    Number of doctoral thesis reviews (chief)
    0
    Number of doctoral thesis reviews (vice-chief)
    1
  • Academic year : 2015

     View Details

    Number of instructed the graduation thesis
    2
    Number of graduation thesis reviews
    0
    Number of instructed the Master course
    8
    Number of instructed the Doctoral course
    1
    Number of master's thesis reviews (chief)
    2
    Number of master's thesis reviews (vice-chief)
    5
    Number of doctoral thesis reviews (chief)
    1
    Number of doctoral thesis reviews (vice-chief)
    1
  • Academic year : 2014

     View Details

    Number of instructed the graduation thesis
    4
    Number of graduation thesis reviews
    0
    Number of instructed the Master course
    5
    Number of instructed the Doctoral course
    2
    Number of master's thesis reviews (chief)
    3
    Number of master's thesis reviews (vice-chief)
    3
    Number of doctoral thesis reviews (chief)
    1
    Number of doctoral thesis reviews (vice-chief)
    0
 

Theme of Possible Research Exchange 【 display / non-display

  • Molecular mechanism of adhesion and gliding in mycoplasmas

    Request for collaborative research : The private sector, such as other institutions

    Type of research exchange : Technical consultation, Consignment study, Collaborative research, Lecture

    Keyword : nanoacutuator, infetious disease, mycoplasma, binding, protein

 

Foreigner acceptance 【 display / non-display

  • Academic year : 2018

     View Details

    Number of foreigners accepted
    1
    Number of International Students
    1

    フランス

  • Academic year : 2017

     View Details

    Number of foreigners accepted
    3
    Number of International Students
    2

    トルコ、アメリカ合衆国(2名)

  • Academic year : 2016

     View Details

    Number of foreigners accepted
    3
    Number of International Students
    3

    トルコ、フランス、中国

  • Academic year : 2015

     View Details

    Number of foreigners accepted
    3
    Number of International Students
    0

    トルコ、フランス、中国

  • Academic year : 2014

     View Details

    Number of foreigners accepted
    4
    Number of International Students
    0