MIYATA Makoto

写真a

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Title

Professor

Laboratory location

Sugimoto Campus

Homepage URL

http://www.sci.osaka-cu.ac.jp/~miyata/myco1.htm

Mail Address

E-mail address

Degree 【 display / non-display

  • Osaka University -  Doctor of Science

Research Areas 【 display / non-display

Biophysics, Bacteriology

Research subject summary 【 display / non-display

  • A bacterial Class, Mollicutes evolved from Phylum Firmicutes to be more specialized to their parasitic life style. They quit peptidoglycan synthesis probably to evade natural immune system of host, resulting in loss of bacterial conventional motility systems such as flagella and pili. Instead, they developed at least three independent motility systems, including two surface motilities.

    Mycoplasma mobile, a fish pathogen form a gliding machinery at a pole and glide on solid surfaces with a speed up to 4.5 μm/s, to the direction of the machinery. We have been studying about structures and components of gliding machinery, binding target, energy source, mechanical characters, and then proposed a working model, "Force generated by a special motor originated from ATP synthase transmits across cell membrane through several proteins, resulting in catch, pull, and release with the sialylated oligosaccharides on host surfaces."

    Since 2012, we are developing the Quick-Freeze Deep-Etch Replica Electron Microscopy method that is useful for studying the motility mechanism, and we are providing this technology to collaborators all over the world.

Research Interests 【 display / non-display

spiroplasma, Protein, mycoplasma, Class mollicutes, Quick freeze replica electron microscopy, Gliding motility, Swimming motility, Motility machinery, Gene

Research Career 【 display / non-display

  • Development of Quick-Freeze Deep-Etch Replica Electron Microscopy method useful for studying motion mechanism

    (Individual) Project Year :

    2012
    -
    Today

  • Mechanism of Spiroplasma swimming movement

    (Individual) Project Year :

    2011
    -
    Today

  • Molecular Mechanism of Gliding Motility of Mycoplasma

    (Individual) Project Year :

    1997
    -
    Today

  • Mycoplasma DNA replication and cell division

    (Individual) Project Year :

    1988
    -
    1997

  • Biochemical study of muscle myosin

    (Individual) Project Year :

    1982
    -
    1988

Association Memberships 【 display / non-display

  • The Biophysical Society of Japan

  • American Society for Microbiology

  • International Organization for Mycoplasmology

  • Japanese Society for mycoplasmology

  • Japanese Society of Bacteriology

Committee Memberships 【 display / non-display

  • 2018.12
    -
    2022.07

    International Organization for Mycoplasmology   Chairperson of IOM's annual meeting in 2022

  • 2018.06
    -
    2020.12

    The Biophysical Society of Japan   Chair of website committee

  • 2018.04
    -
    2019.12

    The Biophysical Society of Japan   Chair of executive committee of annual meeting 2019

  • 2017.06
    -
    Today

    The Biophysical Society of Japan   Board

  • 2017.01
    -
    Today

    Scientific Reports   editorial board member

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Awards & Honors 【 display / non-display

  • Poster award at the 11th Toyota Riken International Workshop on ''Actin Filament: beyond the atomic resolution structures''

    Reconstitution of novel swimming in synthetic bacterium by five bacterial actins

    2019.11.27   Toyota Physical and Chemical Research Institute

    Winner : M1 Hana Kiyama

  • Young researcher Encouragement Award at the 72nd Bacteriology meeting Kansai Branch

    Comparison of gliding motility between type I and type II strains of Mycoplasma pneumoniae

    2019.11.16   Japanese Society for Bacteriology

    Winner : D3 Masaki Mizutani

  • Poster award at the Membrane Symposium 2019

    Analysis of secretion promoting mechanism of Escherichia coli outer membrane vesicles

    2019.11.12   the Membrane society of Japan

    Winner : Ms Tomomi Sawabe (Dr Yoshihiro Ojima and Dr Masayuki Azuma, Graduate school of Engineering, OCU, Collaboration

  • Presentation awards at the 57th annual meeting of the Biophysical society of Japan

    Reconstitution of Spiroplasma eriocheiris swimming motility in a synthetic bacterium

    2019.09.26   Biophysical society of Japan

    Winner : M1 Hana Kiyama

  • Humor Award of the "ABiS Image Contest 2019"

    2019.09.26   Biophysical society of Japan

    Winner : M1 Hiroki Sato

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Current Career 【 display / non-display

  • Osaka City University   Graduate School of Science   Biology and Geosciences Course   Professor  

Career 【 display / non-display

  • 2012.04
    -
    2017.03

    Grand-in-Aid "Harmonized supramolecular motility machinery and its diversity"   Leader

  • 2006.10
    -
    Today

    Osaka City University   Graduate school of Science, Biology & Geosciences,   Professor

  • 2003.10
    -
    2007.03

    PRESTO JST   researcher

  • 2000.03
    -
    2001.03

    Rowland Institute for Science and Harvard University   Visiting Scholar

  • 1998.10
    -
    2006.09

    Osaka City University   Faculty of Science, Department of Biology   assistant professor

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Graduate School 【 display / non-display

  • 1983.04
    -
    1988.03

    Osaka University  Graduate School, Division of Natural Science  physiology 

Graduating School 【 display / non-display

  • 1979.04
    -
    1983.03

    Osaka University   Faculty of Science   Department of Biology

 

Published Papers 【 display / non-display

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Books etc 【 display / non-display

  • Gliding mechanism of Mycoplasma pneumoniae subgroup -implication from Mycoplasma mobile-.

    Miyata M and Nakane D (Part: Joint Work )

    Mollicutes: Molecular Biology and Pathogenesis. Browning G, Citti C (Ed.) Caister Academic Press.  2013

  • Molecular mechanism of mycoplasma gliding - a novel cell motility system.

    Jacques Prost, Kinneret Keren, Herbert Levine, Marileen Dogterom, Sarah Rice, Makoto Miyata, Tanniemola B. Liverpool, Peter Lenz (Part: Joint Work )

    Cell Motility, Lenz P (Ed.), Springer. NewYork  2007.11

     View Summary

    Cell motility is a fascinating example of cell behavior which is fundamentally important to a number of biological and pathological processes. It is based on a complex self-organized mechano-chemical machine consisting of cytoskeletal filaments and molecular motors. In general, the cytoskeleton is responsible for the movement of the entire cell and for movements within the cell. The main challenge in the field of cell motility is to develop a complete physical description on how and why cells move. For this purpose new ways of modeling the properties of biological cells have to be found. This long term goal can only be achieved if new experimental techniques are developed to extract physical information from these living systems and if theoretical models are found which bridge the gap between molecular and mesoscopic length scales. Cell Motility gives an authoritative overview of the fundamental biological facts, theoretical models, and current experimental developments in this fascinating area.

  • Gliding motility of mycoplasmas -A mechanism cannot be explained by current biology-

    Miyata M (Part: Joint Work )

    Mycoplasmas: molecular biology, pathogenecity and strategies for control. Blanchard A, Browning G (Ed.), CRC Press.  2005.04

  • Cell division

    Miyata M (Part: Joint Work )

    Molecular Biology and Pathogenicity of Mycoplasmas. Herrmann R, Razin S (Ed.) Springer, Boston, MA  2002

Review Papers (Misc) 【 display / non-display

  • 【高速AFM観察が可能にした細菌細胞のリアルタイム構造変化】高速AFMが捕らえた!Mycoplasma mobileの滑走装置

    小林 昂平, 古寺 哲幸, 田原 悠平, 豊永 拓真, 笠井 大司, 安藤 敏夫, 宮田 真人

    (公社)日本顕微鏡学会 顕微鏡  54 ( 2 ) 67 - 71 2019.08  [Refereed]  [Invited]

     View Summary

    Mycoplasma mobile(以下モービレ)は、ペプチドグリカン層を持たない、魚の病原細菌(単細胞の生物)である。モービレは固形物表面にはりつき、はりついたまま滑るように動く滑走運動を行う。滑走メカニズムにおいて、モービレの細胞表面にあるタンパク質でできた"あし"が、宿主細胞表面のシアル酸オリゴ糖を引き寄せ、菌体を前に進める。この滑走運動は、細胞内部にあるモーターがATPを加水分解することにより駆動されるが、ATPの加水分解によりモーターがどのような構造変化を起こすかは明らかになっていない。そこで本研究では、高速原子間力顕微鏡(以下高速AFM)を用いて、細胞内におけるモーターの動きを可視化することを目的とした。ガラス基板表面に貼り付けた細胞表面を高速AFMでスキャンすると、内部モーターと思われる粒状の構造がシート状に並んでいる様子が見られた。さらに、個々の粒子は滑走方向に対して右方向に8.2nmシフトする動きを示した。(著者抄録)

  • "Gliding motility of Mycoplasma mobile" - Things seen from the diversity of motility machinery (First part) -

    MIYATA Makoto

    the Society for Biotechnology  96 ( 5 ) 200 - 203 2018.05  [Refereed]  [Invited]

  • For special feature - Things seen from the diversity of motility machinery (the first part) -

    MIYATA Makoto

    the Society for Biotechnology  96 ( 4 ) 182 2018.04  [Refereed]  [Invited]

  • Luminescent Model by Wide-use 3D Printer

    HAMAGUCHI Tasuku, KAWAKAMI Masaru, MIYATA Makoto

    The Biophysical Society of Japan General Incorporated Association, Seibutsu Butsuri  57 ( 4 ) 216 - 218 2017  [Refereed]  [Invited]

    DOI CiNii

  • Following the Random Walk: Howard Berg先生インタビュー

    小嶋 誠司, 政池 知子, 南野 徹, 宮田 真人

    The Biophysical Society of Japan General Incorporated Association 生物物理  54 ( 4 ) 226 - 229 2014

    DOI CiNii

Conference Activities & Talks 【 display / non-display

  • "Structural analysis by electron microscopy for bacteria" Symposium Convener

    Michio Homma, Makoto Miyata  [Invited]

    the 93rd annual meeting of Japanese Society for Bacteriology  (Nagoya)  2020.02  Japanese Society of Bacteriology

  • Supramolecular motility machineries of class Mollicute bacteria

    Makoto Miyata, Takuma Toyonaga, Yuya Sasajima, Takayuki Kato, Akihiro Kawamoto, Tomoko Miyata, Keiichi Namba  [Invited]

    the 93rd annual meeting of Japanese Society for Bacteriology  (Nagoya)  2020.02  Japanese Society of Bacteriology

  • Reconstitution of Spiroplasma swimming and search for its origin in synthetic bacterium, syn3.0

    Hana Kiyama, Shigeyuki Kakizawa, Makoto Miyata  [Invited]

    the 93rd annual meeting of Japanese Society for Bacteriology  (Nagoya)  2020.02  Japanese Society of Bacteriology

  • 合成細菌syn3.0におけるスピロプラズマ遊泳運動の再現とその起源の探索

    木山 花,柿澤 茂行,宮田 真人

    第93回日本細菌学会総会  (名古屋市)  2020.02  日本細菌学会

     View Summary

    Spiroplasma eriocheiris, a crustacean pathogenic bacterium exhibits unique swimming motility by reversing the cell helicity. This swimming motility should be acquired by the developments of five MreBs, actin homologs and Fibril protein, which are specific in the genus Spiroplasma. In this study, we focused on "the synthetic bacterium" created from Mycoplasma with a genome designed with only essential genes. Seven genes including genes encoding the above six proteins were introduced into the synthetic bacterium.
    Surprisingly, the synthetic bacterium originally without any motility showed cell morphology and swimming motility similar to Spiroplasma. Next, with the final goal of elucidating the origin of swimming motility, nonsense mutations were introduced into each of the seven genes. Cells depleted for MreB4 showed changes in the propagation of the helical shift. Cells depleted for MreB5 or MreB2 had a significantly reduced proportion of swimming cells: MreB5 depletion caused many cells with shorter helix pitches, and MreB2 depletion caused a round morphology like a tangled yarn with some motility. We will clarify the minimum set of genes essential for motility by removing more genes. Finally, the origin of motility might be clarified, by substituting Spiroplasma MreBs with ancestral
    proteins.

  • Seroepidemiology on novel colonization factor of enterotoxigenic E. coli O169 in pigs and cattle

    井上 三代子,鄭 冬明,大森 裕子,小松 加奈,山口 良弘,宮田 真人,和田 崇之,麻生 久,中台(鹿毛)枝里子,西川 禎一

    第93回日本細菌学会総会  (名古屋市)  2020.02  日本細菌学会

     View Summary

    Human enterotoxigenic Escherichia coli (ETEC) serotype O169:H41(O169) has been a hugely destructive epidemic ETEC type worldwide; the organisms show massive adherence to HEp-2 cells like enteroaggregative E. coli. Previously, we determined the complete sequence of the unstable virulence plasmid, pEntYN10 (145,082 bp; GC content 46.15% ). Uniquely, the plasmid included a set of genes that encode a novel colonization factor (CF) resembling K88 (F4) of porcine ETEC, beside CS6, a representative CF of human ETEC, and another novel CF similar to CS8 (CFA/III) of human ETEC. The redundancy should affect its epidemiological features and pathogenicity of O169. Using recombinant plasmid with each of three CFs, we revealed that the K88-like enables O169 to adhere to not only human epithelial cells but porcine IPEC and bovine BIE cells in vitro. To estimate whether the K88-like makes O169 infect pigs and cattle, possible adhesin FaeG1 and FaeG2 were extracted from the recombinant organisms transfected with each gene. The seroepidemiological study showed several porcine sera reacted to K88-like FaeG antigens in the western blotting tests. However, a patient serum indicated positive reaction with only CS6. It seems that O169 could select the most suitable CF among three CFs and infect animals except humans.

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Grant-in-Aid for Scientific Research 【 display / non-display

  • Three types of motility mechanisms in pathogenic bacteria, class Mollicutes

    Project/Area Number : 17H01544  Grant-in-Aid for Scientific Research(A) Representative

    Project Year :

    2017.04
    -
    2020.03
     

  • Generalization of harminized supermolecular motility machinery and its diversity

    Project/Area Number : 17H06082  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area) Representative

    Project Year :

    2017.04
    -
    2018.03
     

  • Dynamic bacterial cytoskeleton in Spiroplasma

    Project/Area Number : 15H03712  Grant-in-Aid for Scientific Research(B) Representative

    Project Year :

    2015.04
    -
    2018.03
     

    Partaker : MIYATA Makoto, NAKANE Daisuke

     View Summary

    We have revealed some new aspects on the mechanics of unique swimming motility of bacterium Spiroplasma. In particular, we have constructed the minimal mechanistic model that may explain helical morphologies of Spiroplasma based on the structural biological data on the ribbon-like cytoskeleton. We have also determined the elastic rigidities of Spiroplasma cell by combining theoretical and numerical analysis with the data from the micromanipulation experiments. In addition to these, we have studied fundamental geometric mechanical properties of macro-scale elastic ribbons by combining theory and experiment, and discussed our new findings in relation to cellular mechanics of bacteria. To summarize, our achievement during the period of this project will significantly contribute to the understanding of yet mysterious mechanism of Spiroplasma swimming dynamics.

  • Mechanism of Mycoplasma gliding

    Project/Area Number : 24117002  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area) Representative

    Project Year :

    2012.06
    -
    2017.03
     

  • Harmonized supramolecular machinery for motility and its diversity

    Project/Area Number : 3407  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area) Representative

    Project Year :

    2012.06
    -
    2017.03
     

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Charge of on-campus class subject 【 display / non-display

  • Basic Medical Sciences Program

    (2019) University, Special course

  • Biological Science

    (2019) University, Special course

  • Advanced Laboratory in Biology A

    (2019) University, Special course

  • Senior Thesis

    (2019) University, Special course

  • Advanced Molecular Cell Biology II

    (2019) Graduate school, Special course

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Educational activity record 【 display / non-display

  • Academic year : 2019

     View Details

    Number of instructed the graduation thesis
    4
    Number of graduation thesis reviews
    0
    Number of instructed the Master course
    5
    Number of instructed the Doctoral course
    4
    Number of master's thesis reviews (chief)
    3
    Number of master's thesis reviews (vice-chief)
    4
    Number of doctoral thesis reviews (chief)
    1
    Number of doctoral thesis reviews (vice-chief)
    0
  • Academic year : 2018

     View Details

    Number of instructed the graduation thesis
    2
    Number of graduation thesis reviews
    0
    Number of instructed the Master course
    3
    Number of instructed the Doctoral course
    4
    Number of master's thesis reviews (chief)
    0
    Number of master's thesis reviews (vice-chief)
    5
    Number of doctoral thesis reviews (chief)
    0
    Number of doctoral thesis reviews (vice-chief)
    0
  • Academic year : 2017

     View Details

    Number of instructed the graduation thesis
    3
    Number of graduation thesis reviews
    0
    Number of instructed the Master course
    2
    Number of instructed the Doctoral course
    4
    Number of master's thesis reviews (chief)
    2
    Number of master's thesis reviews (vice-chief)
    6
    Number of doctoral thesis reviews (chief)
    0
    Number of doctoral thesis reviews (vice-chief)
    0
  • Academic year : 2016

     View Details

    Number of instructed the graduation thesis
    3
    Number of instructed the Master course
    7
    Number of instructed the Doctoral course
    0
    Number of master's thesis reviews (chief)
    5
    Number of master's thesis reviews (vice-chief)
    4
    Number of doctoral thesis reviews (chief)
    0
    Number of doctoral thesis reviews (vice-chief)
    1
  • Academic year : 2015

     View Details

    Number of instructed the graduation thesis
    2
    Number of graduation thesis reviews
    0
    Number of instructed the Master course
    8
    Number of instructed the Doctoral course
    1
    Number of master's thesis reviews (chief)
    2
    Number of master's thesis reviews (vice-chief)
    5
    Number of doctoral thesis reviews (chief)
    1
    Number of doctoral thesis reviews (vice-chief)
    1

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Theme of Possible Research Exchange 【 display / non-display

  • Molecular mechanism of adhesion and gliding in mycoplasmas

    Request for collaborative research : The private sector, such as other institutions

    Type of research exchange : Technical consultation, Consignment study, Collaborative research, Lecture

    Keyword : nanoacutuator, infetious disease, mycoplasma, binding, protein

 

Foreigner acceptance 【 display / non-display

  • Academic year : 2019

     View Details

    Number of foreigners accepted
    0
    Number of International Students
    1
  • Academic year : 2018

     View Details

    Number of foreigners accepted
    1
    Number of International Students
    1

    フランス

  • Academic year : 2017

     View Details

    Number of foreigners accepted
    3
    Number of International Students
    2

    トルコ、アメリカ合衆国(2名)

  • Academic year : 2016

     View Details

    Number of foreigners accepted
    3
    Number of International Students
    3

    トルコ、フランス、中国

  • Academic year : 2015

     View Details

    Number of foreigners accepted
    3
    Number of International Students
    0

    トルコ、フランス、中国

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