NAGASAKI Takeshi

写真a

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Title

Professor

Laboratory location

Sugimoto Campus

Mail Address

E-mail address

Degree 【 display / non-display

  • Kyushu University -  Doctor of Engineering

Research Areas 【 display / non-display

Medical Bioengineering, Biofunctional Engineering

Research Interests 【 display / non-display

Drug Delivery System, Neutron Capture Therapy, Anti-cancer agent, Cell Scaffold, Tissue Adhesive

Research Career 【 display / non-display

  • Boron Delivery System for Boron Neutron Capture Therapy

    (Collaboration in Japan) Project Year :

    2010.04
    -
    Today

  • Hydrogel as Hemostat

    (Collaboration in Japan) Project Year :

    2006.04
    -
    Today

    Keyword in research subject:  Hemostat, Hydro Gel

  • Controlled Drug Delivery by Using Functional Protein

    (Collaboration in Organization) Project Year :

    2000.04
    -
    Today

    Keyword in research subject:  drug delivery, intracellular trafficking, functional protein

  • Development of Cell Culutre Devices

    (Collaboration in Japan) Project Year :

    2003.04
    -
    Today

    Keyword in research subject:  cell culture, polycation, cell adhesion

  • Biopolymer-based gene carrier

    (Collaboration in Japan) Project Year :

    1999.04
    -
    Today

    Keyword in research subject:  gene carrier, biopolymer, polycation

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Committee Memberships 【 display / non-display

  • 2008
    -
    Today

    Association of Research for Host-Guest and Supramolecular Chemistry   Board

  • 2000
    -
    Today

    The Japanese Society of Gene Design and Delivery   Board

Awards & Honors 【 display / non-display

  • Bull. Chem. Soc. Jpn. Award

    2007  

  • HGCS Japan Award of Excellence 2006

    2006  

  • Bull. Chem. Soc. Jpn. Award

    2004  

Current Career 【 display / non-display

  • Osaka City University   Graduate School of Engineering   Applied Chemistry and Bioengineering Course   Professor  

  • Osaka City University   Graduate School of Engineering   Dean  

Career 【 display / non-display

  • 2007.04
     
     

    Osaka City University  

  • 1995.04
     
     

    Osaka City University  

  • 1994.04
     
     

    Osaka City University  

  • 1991
    -
    1994

    Faculty of Engineering, Kyushu University   Research Associate

Graduate School 【 display / non-display

  • 1988.04
    -
    1991.03

    Kyushu University  Graduate School, Division of Engineering  Department of Organic Synthesis 

  • 1986.04
    -
    1988.03

    Tokyo University of Agriculture and Technology  Graduate School, Division of Engineering  Department of Applied Chemistry for Resources 

Graduating School 【 display / non-display

  • 1982.04
    -
    1986.03

    Tokyo University of Agriculture and Technology   Faculty of Engineering   Department of Applied Chemistry for Resources

 

Published Papers 【 display / non-display

  • HVJ-E/importin-b hybrid vector for overcoming cytoplasmic and nuclear membranes as double barrier for non-viral gene delivery

    Takeshi Kawazu, Hiroyuki Kanzaki, Atsushi Uno, Hideki Azuma, Takeshi Nagasaki

    Elsevier  66 ( 7 ) 519 - 524 2012.07  [Refereed]

  • Photochemical controlof membrane raft organization

    Soft Matter  7   220 - 224 2011

  • Improved Stability and Photodynamic Activity of Water-Soluble 5,15-Diazaporphyrins Incorporated in beta-(1,3-1,6)-d-Glucan with On-Off Switch

    Hino Shodai, Satake Shuhei, Shinmori Hideyuki, Kawabata Shigeki, Koumoto Kazuya, Suzuki Toshio, Nagasaki Takeshi, Sugikawa Kouta, Kawasaki Riku, Ikeda Atsushi

    CHEMISTRY-AN ASIAN JOURNAL  15 ( 3 ) 365 - 370 2020.02  [Refereed]

    DOI PubMed

  • Turn-on fluorescence and photodynamic activity of beta-(1,3-1,6)-d-glucan-complexed porphyrin derivatives inside HeLa cells

    Hino Shodai, Funada Rikushi, Sugikawa Kouta, Koumoto Kazuya, Suzuki Toshio, Nagasaki Takeshi, Ikeda Atsushi

    PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES  18 ( 12 ) 2854 - 2858 2019.12  [Refereed]

    DOI PubMed

  • Formation of beta-(1,3-1,6)-D-glucan-complexed [70]fullerene and its photodynamic activity towards macrophages (vol 15, pg 1990, 2017)

    Ikeda Atsushi, Akiyama Motofusa, Sugikawa Kouta, Koumoto Kazuya, Kagoshima Yuta, Li Jiawei, Suzuki Toshio, Nagasaki Takeshi

    ORGANIC & BIOMOLECULAR CHEMISTRY  15 ( 45 ) 9734 - 9734 2017.12  [Refereed]

    DOI

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Books etc 【 display / non-display

  • Ultrasound-mediated Transfection with Liposomal Bubbles Delivers Plasmid DNA Directly into Nucleu

    Takeshi Kawazu, Kazumi Hakamada, Yusuke Oda, Jun Miyake, Kazuo Maruyama, and Takeshi Nagasaki (Part: Joint Work )

    Chem. Lett.  2011.03

  • Bottom-Up Nanofablication, Development of Molecular Machinery as Nonviral Vectors

    Tomomi Jinta, Takeshi Nagasaki (Part: Contributor )

    American Scientific Publishers  2009

  • Non-viral Gene Therapy: Gene Design and Derivery, 2.2 Active DNA Release from Complexes

    (Part: Single Work )

    Springer-Verlag, Tokyo  2005

  • Non-viral Gene Therapy: Gene Design and Derivery, 2.4 Active Transport of Exogenous Genes into the Nucleus

    (Part: Joint Work )

    Springer-Verlag, Tokyo  2005

Review Papers (Misc) 【 display / non-display

  • Photoresponsive Gene Delivery System: Photoregulation of Intracellular Trafficking Using Photoresponsive Liposome

    Takeshi Nagasaki, Tsutomu Hamada

    Laser Society of Japan  45 ( 11 ) 696 - 700 2017.11  [Refereed]  [Invited]

     View Summary

    Since light as a stimulus can be controlled both temporally and spatially, photo-irradiation improves both the endosomal escape of exogenous DNA and DNA release from the complexes with carriers. When a photo-responsive cationic lipid formed a cell-sized giant vesicle, we observed the photo-isomerization effect of azobenzene on its shape change. Photoswitching the membrane line tension induced reversible transitions between a closed sphere and an opened disk with a bilayer pore. In a transfection experiment using a photoresponsive cationic lipid, UV irradiation improved the transfection efficiency. After the lipoplexes passed through the plasma membrane by endocytosis, trans to cis isomerization of azobenzene moiety destabilized the vesicle membrane, thus accelerating not only the membrane fusion of the photo-responsive lipid vesicles but also the endosomal escape of the exogenous DNA.

  • 光に応答する遺伝子デリバリーシステム 〜エンドソーム脱出を中心とした細胞内トラフィッキング光制御〜

    長崎 健, 濱田 勉

    レーザー研究  45 ( 11 ) 696 - 700 2017.11  [Refereed]  [Invited]

     View Summary

    アゾベンゼンを基体とする光応答性脂質はtrans- cisの構造変化によりその分子集合体の形態が大きく変化することが明らかとなった.この変化は光異性化による膜の物性変化に基づく.またこの変化により他の二分子膜との融合などが誘起され,エンドサイトーシスにより細胞内に取り込まれた場合にはエンドソーム膜との融合により,薬物の細胞内輸送にも大きな影響を与えることが明らかとなった.実際,光開裂性脂質を用いた時,蛍光ラベル化複合体の蛍光顕微鏡観察によりエンドソーム脱出の光促進が確認出来た.

  • 健康・医療用マテリアルとしてのβ-1,3-グルカン

    鈴木利雄, 長崎 健

    機能材料  30 ( 9 ) 54 - 62 2010  [Refereed]  [Invited]

  • The Concept of Molecular Machinery Is Useful for Design of Stimuli-Responsive Gene Delivery Systems in the Mammalian Cell.

    J. Incl. Phenom. Macrocycl. Chem.  58 ( 3-4 ) 205 - 219 2007

  • Extraction Behavior of Copper Ion by Calixarene Carboxylate Derivatives Preorganized by Sodium Ion((Multiple Authorship))

    Solvent Extraction and Ion Exchange  14 ( 3 ) 459 - 478 1996

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Conference Activities & Talks 【 display / non-display

  • Cellular uptake mechanism of kojic acid modified o-carborane as a boron drug toward melanoma-targeting BNCT

    Takeshi Nagasaki, Satoshi Dowaki , Riku Kawasaki , Koki Matsuura , Yoshihide Hattori, Yoshinori Sakurai, Shin-ichiro Masunaga, Mitsunori Kirihata

    10th Young Researchers BNCT Meeting  2019.09 

  • Foundation of Educational Research Center of Graduate School of Engineering, Osaka City University

     [Invited]

    2019.02 

  • Functional evaluation of kojic acid modified carborane developed as a boron drug for melanoma BNCT

    Satoshi DOWAKI, Koki MATSUURA, Riku KAWASAKI, Yoshihide HATTORI, Yoshinori SAKURAI, Shinichiro MASUNAGA, Mitsunori KIRIHATA, Takeshi NAGASAKI

    18th International Congress on Neutron Capture Therapy  2018.11 

  • Suppression of Melanoma Metastasis by Kojic Acid Modified Carborane

    Satoshi Dowaki、Koki Matsuura、Riku Kawasaki、Yoshihide Hattori、Yoshinori Sakurai、Hiroki Tanaka、Shin-ichiro Masunaga、Mitsunori kirihata、Takeshi Nagasaki

    9th Young Researchers' BNCT Meeting  (京大おうばくプラザ)  2017.11 

  • Physical properties and effect of glucose concentration responsive injectable hydrogel

    Takuya Mukaeda、Yu-I Hsu、Takeshi Nagasaki、 Tetsuji Yamaoka

    6th Asian Biomaterials Congress  (Appolo DIMORA, Thiruvananthapuram, India)  2017.10 

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Grant-in-Aid for Scientific Research 【 display / non-display

  • Development of Inorganic Boron Cluster-fused Delivery Materials for the Neutron Capture Therapy

     Representative

    Project Year :

    2011.04
    -
    2013.03
     

  • Development of multifunctional vectors

    Grant-in-Aid for Scientific Research(C) Representative

    Project Year :

    2005.04
    -
    2007.03
     

  • Study on interaction between the photoresponsive dendrimer and biorelative compounds

    Grant-in-Aid for Scientific Research(C) Representative

    Project Year :

    1997.04
    -
    2000.03
     

 

Other educational activity and Special note 【 display / non-display

  • Contribution to University-wide Cross-training program

    (2018)

  • Contribution to FD activities

    (2010)

Educational activity record 【 display / non-display

  • Academic year : 2010

     View Details

    Number of instructed the graduation thesis
    3
    Number of graduation thesis reviews
    3
    Number of instructed the Master course
    5
    Number of instructed the Doctoral course
    2
    Number of master's thesis reviews (chief)
    5
    Number of master's thesis reviews (vice-chief)
    4
    Number of doctoral thesis reviews (chief)
    0
    Number of doctoral thesis reviews (vice-chief)
    0
 

Theme of Possible Research Exchange 【 display / non-display

  • Development of cell culture devise

    Request for collaborative research : The private sector, such as other institutions

    Type of research exchange : Technical consultation, Consignment study, Collaborative research, Lecture

    Keyword : cell culture, polycation, cell adhesion

  • Development of biopolymer-based gene carrier

    Request for collaborative research : The private sector, such as other institutions

    Type of research exchange : Technical consultation, Consignment study, Collaborative research, Lecture

    Keyword : transfection, biopolymer, biodegradability, polycation, liposome

  • Controlled drug delivery by using functional protein

    Request for collaborative research : The private sector, such as other institutions

    Type of research exchange : Technical consultation, Consignment study, Collaborative research, Lecture

    Keyword : drug delivery, intracellular trafficking

 

Foreigner acceptance 【 display / non-display

  • Academic year : 2017

     View Details

    Number of International Students
    1
  • Academic year : 2010

     View Details

    Number of foreigners accepted
    1
    Number of International Students
    2