KOYANAGI Mitsumasa

写真a

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Title

Professor

Laboratory location

Sugimoto Campus

Mail Address

E-mail address

Degree 【 display / non-display

  • Kyoto University -  D.sci

Research Areas 【 display / non-display

Photobiology, Molecular Evolution

Research Interests 【 display / non-display

Photobiology, Evolution, Optogenetics, Non-visual photoreception, Photoreceptor, GPCR, Sensory system, Light sensing, Color vision, Vision

Research Career 【 display / non-display

  • Origin and diversity of the animal photoreception.

    (Individual) Project Year :

    2005.04
     
     

    Keyword in research subject:  photoreception, rhodopsin

  • Functional analyses of non-image-forming color vision.

    (Individual) Project Year :

    2006.04
     
     

    Keyword in research subject:  non-visual, rhodopsin, color vision

Association Memberships 【 display / non-display

  • THE ZOOLOGICAL SOCIETY OF JAPAN

Awards & Honors 【 display / non-display

  • JSPS Award

    2017  

  • Promotion Award of the Zoological Society of Japan

    2013  

    Winner : KOYANAGI Mitsumasa

  • Nature Industry Award of Osaka Science and Technology Center

    2012  

  • Promotion Award of the Photobiology Association of Japan

    2012  

  • Yoshida Prize for Young Researchers of the Japanese Society for Comparative Physiology and Biochemistry

    2006  

Current Career 【 display / non-display

  • Osaka City University   Graduate School of Science   Biology and Geosciences Course   Professor  

  • Osaka City University   The OCU Advanced Research Institute for Natural Science and Technology (OCARINA)   Researcher  

Career 【 display / non-display

  • 2019.10
    -
    Today

    Osaka City University   Graduate School of Science   Professor

  • 2015.04
    -
    Today

    Osaka City University   OCARINA   Researcher (Concurrent)

  • 2013.10
    -
    2017.03

    JST   PRESTO Researcher (Concurrent)

  • 2010.10
    -
    2019.09

    Osaka City University   Graduate School of Science   Associate Professor

  • 2006.04
    -
    2010.09

    Osaka City University   Graduate School of Science   Lecturer

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Graduate School 【 display / non-display

  • 1997.04
    -
    2001.03

    Kyoto University  Graduate School, Division of Natural Science 

  • 1995.04
    -
    1997.03

    Kyoto University  Graduate School, Division of Natural Science 

Graduating School 【 display / non-display

  • 1991.04
    -
    1995.03

    Kyoto University   Faculty of Science  

 

Published Papers 【 display / non-display

  • Functional identification of an opsin kinase underlying inactivation of the pineal bistable opsin parapinopsin in zebrafish

    Shen Baoguo, Wada Seiji, Nishioka Haruka, Nagata Takashi, Kawano-Yamashita Emi, Koyanagi Mitsumasa, Terakita Akihisa

    ZOOLOGICAL LETTERS  7 ( 1 )  2021.02  [Refereed]

    DOI

  • Optogenetic Potentials of Diverse Animal Opsins: Parapinopsin, Peropsin, LWS Bistable Opsin

    Koyanagi Mitsumasa, Saito Tomoka, Wada Seiji, Nagata Takashi, Kawano-Yamashita Emi, Terakita Akihisa

    OPTOGENETICS: LIGHT-SENSING PROTEINS AND THEIR APPLICATIONS IN NEUROSCIENCE AND BEYOND, 2ND EDITION  1293   141 - 151 2021.01  [Refereed]

    DOI

  • Visual and nonvisual opsin genes of sharks and other nonosteichthyan vertebrates: Genomic exploration of underwater photoreception

    Yamaguchi Kazuaki, Koyanagi Mitsumasa, Kuraku Shigehiro

    JOURNAL OF EVOLUTIONARY BIOLOGY  2020.11  [Refereed]

     View Summary

    Vision of sharks embraces various biological and ecological themes ranging from predation and adaptation to deep-sea life. However, behavioural and genetic studies have been limited by their elusive lifestyles, repeatedly reported declines of wild populations, and their unique life-history traits including low fecundity and enhanced longevity. Sharks have also not been actively studied on the cellular and molecular levels, because of additional difficulties in cell culture, tissue collection and genome sequencing. A recent study circumvented some of these obstacles by means of genome informatics thereby portrayed the variation of visual opsin gene repertoires among elasmobranchs (sharks and rays) and spectral shifts of the rhodopsin pigment. Comprehensive surveys in whole-genome sequences are also revealing the repertoires of nonvisual opsins with unknown functions. This review is aimed to summarize existing studies on shark opsins with an emphasis on genomic investigation of gene repertoires and to provide insights into the better understanding of underwater ecology of marine megafauna with in vitro experimentation.

    DOI PubMed

  • From extraocular photoreception to pigment movement regulation: a new control mechanism of the lanternshark luminescence

    Duchatelet Laurent, Sugihara Tomohiro, Delroisse Jerome, Koyanagi Mitsumasa, Rezsohazy Rene, Terakita Akihisa, Mallefet Jerome

    SCIENTIFIC REPORTS  10 ( 1 ) 10195 - 10195 2020.06  [Refereed]

     View Summary

    The velvet belly lanternshark, Etmopterus spinax, uses counterillumination to disappear in the surrounding blue light of its marine environment. This shark displays hormonally controlled bioluminescence in which melatonin (MT) and prolactin (PRL) trigger light emission, while α-melanocyte-stimulating hormone (α-MSH) and adrenocorticotropic hormone (ACTH) play an inhibitory role. The extraocular encephalopsin (Es-Opn3) was also hypothesized to act as a luminescence regulator. The majority of these compounds (MT, α-MSH, ACTH, opsin) are members of the rapid physiological colour change that regulates the pigment motion within chromatophores in metazoans. Interestingly, the lanternshark photophore comprises a specific iris-like structure (ILS), partially composed of melanophore-like cells, serving as a photophore shutter. Here, we investigated the role of (i) Es-Opn3 and (ii) actors involved in both MT and α-MSH/ACTH pathways on the shark bioluminescence and ILS cell pigment motions. Our results reveal the implication of Es-Opn3, MT, inositol triphosphate (IP3), intracellular calcium, calcium-dependent calmodulin and dynein in the ILS cell pigment aggregation. Conversely, our results highlighted the implication of the α-MSH/ACTH pathway, involving kinesin, in the dispersion of the ILS cell pigment. The lanternshark luminescence then appears to be controlled by the balanced bidirectional motion of ILS cell pigments within the photophore. This suggests a functional link between photoreception and photoemission in the photogenic tissue of lanternsharks and gives precious insights into the bioluminescence control of these organisms.

    DOI PubMed

  • The non-visual opsins expressed in deep brain neurons projecting to the retina in lampreys

    Kawano-Yamashita Emi, Koyanagi Mitsumasa, Wada Seiji, Saito Tomoka, Sugihara Tomohiro, Tamotsu Satoshi, Terakita Akihisa

    SCIENTIFIC REPORTS  10 ( 1 ) 9669 - 9669 2020.06  [Refereed]

     View Summary

    In lower vertebrates, brain photoreceptor cells express vertebrate-specific non-visual opsins. We previously revealed that a pineal-related organ-specific opsin, parapinopsin, is UV-sensitive and allows pineal wavelength discrimination in lampreys and teleost. The Australian pouched lamprey was recently reported as having two parapinopsin-related genes. We demonstrate that a parapinopsin-like opsin from the Japanese river lamprey exhibits different molecular properties and distribution than parapinopsin. This opsin activates Gi-type G protein in a mammalian cell culture assay in a light-dependent manner. Heterologous action spectroscopy revealed that the opsin forms a violet to blue-sensitive pigment. Interestingly, the opsin is co-localised with green-sensitive P-opsin in the cells of the M5 nucleus of Schober (M5NS) in the mesencephalon of the river and brook lamprey. Some opsins-containing cells of the river lamprey have cilia and others an axon projecting to the retina. The opsins of the brook lamprey are co-localised in the cilia of centrifugal neurons projecting to the retina, suggesting that cells expressing the parapinopsin-like opsin and P-opsin are sensitive to violet to green light. Moreover, we found neural connections between M5NS cells expressing the opsins and the retina. These findings suggest that the retinal activity might be modulated by brain photoreception.

    DOI PubMed

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Books etc 【 display / non-display

  • Optogenetics : light-sensing proteins and their applications

    八尾 寛, 神取 秀樹, 小泉 周 (Part: Joint Work )

    Springer  2015

  • Evolution of visual and non-visual pigments

    Hunt David M. (Part: Joint Work )

    Springer  2014

  • 昆虫とクモの仲間

    針山 孝彦, 小柳 光正, 嬉 正勝, 妹尾 圭司, 小泉 修, 日本比較生理生化学会 (Part: Single Work )

    共立出版  2012

    CiNii

  • ゾウリムシ,ヒドラ,貝,エビなど

    針山 孝彦, 小柳 光正, 嬉 正勝, 妹尾 圭司, 小泉 修, 日本比較生理生化学会 (Part: Single Work )

    共立出版  2012

    CiNii

  • ウニ,ナマコから脊椎動物へ

    針山 孝彦, 小柳 光正, 嬉 正勝, 妹尾 圭司, 小泉 修, 日本比較生理生化学会 (Part: Single Work )

    共立出版  2012

    CiNii

Review Papers (Misc) 【 display / non-display

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Conference Activities & Talks 【 display / non-display

  • Toward a new method for selective activation of a non-visual opsin

    Makoto Iwasaki, Tomohiro Sugihara, Seiji Wada, Mitsumasa Koyanagi, Akihisa Terakita

    日本比較生理生化学会第42回山形大会  2020.11 

  • Investigation of opsins in the reef-building coral, Acropora tenuis, exhibiting a light-response behavior at the larval stage

    Yusuke Sakai, Hisao Tsukamoto, Naoto Ueno, Mitsumasa Koyanagi, Akihisa Terakita

    日本比較生理生化学会第42回山形大会  2020.11 

  • Investigation of opsin-like GPCRs identified in a primitive multicellular animal

    Naoki Takahashi, Akihisa Terakita, Mitsumasa Koyanagi

    日本比較生理生化学会第42回山形大会  2020.11 

  • Histological investigation of deep brain photoreceptors in the larval and adult lampreys

    Emi Kawano-Yamashita, Nanaho Yura, Mitsumasa Koyanagi, Seiji Wada, Akihisa Terakita, Satoshi Tamotsu

    日本比較生理生化学会第42回山形大会  2020.11 

  • Functional analysis of melaonpsin expressed in retinal horizontal cells of zebrafish

    Momoka Mano, Seiji Wada, Mitsumasa Koyanagi, Akihisa Terakita

    日本比較生理生化学会第42回山形大会  2020.11 

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Grant-in-Aid for Scientific Research 【 display / non-display

  • A new strategy for investigating GPCR-based physiologies by using rhodopsins

    Project/Area Number : 20K21433  Grant-in-Aid for Challenging Research (Exploratory) Representative

    Project Year :

    2020.07
    -
    2023.03
     

  • Study on a non-visual photoreception lost in the course of mammalian evolution

    Project/Area Number : 15K14568  Grant-in-Aid for challenging Exploratory Research Representative

    Project Year :

    2015.04
    -
    2017.03
     

    Partaker : TERAKITA Akihisa

     View Summary

    We discovered a photoreceptor gene (opsin), which does not exist in eutherian genomes, in marsupial genomes. In this study, we carried out functional analyses of the marsupial opsin spectroscopically and biochemically to understand the diversity of photoreception in mammals. We found that the marsupial opsin indeed forms a photosensitive pigment. In addition, histochemical analysis revealed that the marsupial opsin exhibits a different expression pattern from other opsins in opossum, suggesting a novel photoreception in mammals.

  • Investigation of the diversity of visual and non-visual UV reception in jumping spiders

    Project/Area Number : 26291070  Grant-in-Aid for Scientific Research(B) Representative

    Project Year :

    2014.04
    -
    2018.03
     

  • Functional analysis of genetic variation in melanoposin, a non-visual photoreceptor protein for circadian photoentrainment and its implication for phenotype

    Project/Area Number : 23657175  Grant-in-Aid for challenging Exploratory Research Representative

    Project Year :

    2011
    -
    2012
     

     View Summary

    We succeeded in a large scale expression of a mammalian melanopsin, a circadian photoreceptor protein, and its polymorphic variant in cultured cells, and elucidated photochemical and biochemical characteristics of them. We also

  • Investigation of the pineal wavelength discrimination in lower vertebrates

    Project/Area Number : 22687005  Grant-in-Aid for Young Scientists(A) Representative

    Project Year :

    2010.04
    -
    2014.03
     

     View Summary

    In lower vertebrates, pineal and related organs detect the ratio of UV to visible lights, suggesting that wavelengths of environmental light are discriminated by these organs independently of eyes. Recently, we found that parapinopsin is a UV sensitive photopigment that underlies the UV reception for wavelength discrimination in the lamprey pineal. Here, we carried out the parapinopsin-based investigation of the pineal wavelength discrimination in varied lower vertebrates and revealed the diversity and generality of the wavelength discrimination mechanism. Furthermore, by using transgenic technique, we obtained a clue to the physiological roles of the pineal wavelength discrimination. We also found that a homologue of another non-visual photopigment Opn3 has an unique characteristic for functioning as a light sensor in extraocular tissues.

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Other educational activity and Special note 【 display / non-display

  • Contribution to FD activities

    (2020)

  • Contribution to FD activities

    (2019)

  • Contribution to FD activities

    (2018)

  • Contribution to FD activities

    (2017)

 

Foreigner acceptance 【 display / non-display

  • Academic year : 2020

     View Details

    Number of foreigners accepted
    0
    Number of International Students
    1
  • Academic year : 2019

     View Details

    Number of foreigners accepted
    0
    Number of International Students
    1
  • Academic year : 2018

     View Details

    Number of foreigners accepted
    1
    Number of International Students
    1

    United Kingdom

  • Academic year : 2017

     View Details

    Number of foreigners accepted
    1
    Number of International Students
    1

    Germany